Didilescu Andreea C, Skaug Nils, Marica Constantin, Didilescu Cristian
Department of Anatomy and Embryology, Faculty of Dental Medicine, University of Medicine and Pharmacy Carol Davila, Bucharest, Romania.
Clin Oral Investig. 2005 Sep;9(3):141-7. doi: 10.1007/s00784-005-0315-6. Epub 2005 May 21.
Bacterial cultivation studies have shown that dental plaque is a reservoir for respiratory pathogens in intensive care unit patients and in elderly who are debilitated, hospitalized or in a nursing home, placing them at risk of bacterial pneumonia. No information is available, however, concerning dental plaque as a reservoir of putative respiratory pathogens in hospitalized patients with chronic lung diseases. Supragingival plaque colonization of 34 hospitalized chronic lung-diseased Romanian citizens, excluding those with tuberculosis and less than 20 teeth, was therefore assessed by checkerboard DNA-DNA hybridization using a selected panel of whole genomic DNA probes produced from eight respiratory pathogens and eight oral pathogens. Thirty-one lung-healthy dental outpatients served as reference population. Respiratory pathogens were detected in plaque from 29 of the 34 (85.3%) hospitalized patients and 12 of the 31 (38.7%) reference population subjects. Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, and Enterobacter cloacae occurred significantly (p<0.05) more frequent among the hospitalized patients. Hospitalized chronic lung-diseased patients harbored in their supragingival plaque samples bacteria known to cause nosocomial pneumonia significantly (p<0.001) more frequent than lung-healthy dental outpatients. Our results indicate that dental plaque in patients with chronic lung diseases often serves as a reservoir of bacteria known to cause nosocomial pneumonia in susceptible individuals.
细菌培养研究表明，牙菌斑是重症监护病房患者以及体弱、住院或住在疗养院的老年人呼吸道病原体的储存库，使他们面临细菌性肺炎的风险。然而，关于牙菌斑作为慢性肺病住院患者潜在呼吸道病原体储存库的信息却尚无定论。因此，我们使用从8种呼吸道病原体和8种口腔病原体中提取的一组全基因组DNA探针，通过棋盘式DNA-DNA杂交技术，对34名住院的罗马尼亚慢性肺病患者（不包括患有结核病和牙齿少于20颗的患者）的龈上菌斑定植情况进行了检测评价。3１名肺部健康的牙科门诊患者作为对照人群。在34名住院患者中的29位患者(8５.3%)以及31名对照人群中的12位(３8.7%)的菌斑中检测到了呼吸道病原体。金黄色葡萄球菌、铜绿假单胞菌、鲍曼不动杆菌和阴沟肠杆菌在住院患者中出现频率显著更高(ｐ<0.05)。住院慢性肺病患者龈上菌斑样本中已知可引起医院获得性肺炎的细菌携带率显著高于肺部健康的牙科门诊患者(ｐ<0.001)。我们的研究结果表明，慢性肺病患者的牙菌斑常作为已知可在易感个体中引起医院获得性肺炎的细菌的储存库。
