Nataraj Mounithaa, Carmelin Durai Singh, Geetha Sravanthy P, Saravanan Muthupandian
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, IND.
Cureus. 2024 Apr 15;16(4):e58350. doi: 10.7759/cureus.58350. eCollection 2024 Apr.
Background The evolution of new respiratory diseases, especially upper respiratory tract infections and resistance of pathogens to various antibiotic treatments, needs an alternative way of medication. Chronic respiratory infections in both adults and infants are the major cause of morbidity and mortality, particularly in developing countries. The widespread application of nanomaterials in the field of medicine and the incorporation of nanoparticles in drugs are taken into account. These nanomaterials are involved along with the biosynthesis of plant extract. In this study, selenium oxide nanoparticles (SeO-NPs), known as a significant trace element for human health, were synthesized in an eco-friendly manner. Methodology Green synthesis of -mediated SeO-NPs was proceeded by titration method and nanoparticles were synthesized. The color intensity, morphological characters, functional properties, and involvement of phytochemical compounds were studied by using UV-visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD), scanning electron microscope (SEM), and energy-dispersive X-ray spectroscopy (EDX) analysis. Results The synthesized extract showed a color change from brown to ruby red. Results obtained by characterization and biological assays depicted that the Centella asiatica-mediated SeO-NPs showed absorbance at the peak level 320 nm by UV-Vis spectroscopy, several phytochemical compounds, and O-H functional groups by FT-IR which may be involved in the reduction of the selenium oxide nanoparticles. The XRD showed 57.1% crystalline and 42.6% amorphous nature. The SEM images showed that agglomerated spherical shapes were involved in biological activities. The EDX analysis showed the presence of Se, C, and O compounds. Further, the antibacterial activity of the synthesized nanoparticles showed significant activity in the multidrug-resistant respiratory pathogens. Conclusions Based on the characterization studies and biomedical assays, it can be concluded that the incorporation of SeO-NPs along with the plant extract serves as the best remedy and organic treatment for upper respiratory tract infections. We plan to conduct further in-vivo, toxicity-level studies, and clinical trials.
背景 新型呼吸道疾病的演变，尤其是上呼吸道感染以及病原体对各种抗生素治疗的耐药性，需要一种替代的药物治疗方式。成人和婴儿的慢性呼吸道感染是发病和死亡的主要原因，在发展中国家尤为如此。考虑到纳米材料在医学领域的广泛应用以及纳米颗粒在药物中的掺入。这些纳米材料与植物提取物的生物合成相关。在本研究中，以生态友好的方式合成了对人体健康至关重要的微量元素氧化硒纳米颗粒（SeO-NPs）。
方法 通过滴定法进行 -介导的SeO-NPs的绿色合成并制备纳米颗粒。利用紫外可见光谱（UV-Vis）、傅里叶变换红外光谱（FT-IR）、X射线粉末衍射（XRD）、扫描电子显微镜（SEM）和能量色散X射线光谱（EDX）分析研究了颜色强度、形态特征、功能特性以及植物化学化合物的参与情况。
结果 合成的提取物颜色从棕色变为红宝石红色。表征和生物学测定结果表明，积雪草介导的SeO-NPs通过紫外可见光谱在320 nm峰值水平处有吸光度，通过傅里叶变换红外光谱有几种植物化学化合物和O-H官能团，这些可能参与了氧化硒纳米颗粒的还原。X射线粉末衍射显示57.1%为结晶性质，42.6%为无定形性质。扫描电子显微镜图像显示团聚的球形参与了生物活性。能量色散X射线光谱分析表明存在Se、C和O化合物。此外，合成的纳米颗粒对多重耐药呼吸道病原体具有显著的抗菌活性。
结论 基于表征研究和生物医学测定，可以得出结论，SeO-NPs与植物提取物的结合是上呼吸道感染的最佳治疗方法和有机疗法。我们计划进一步进行体内、毒性水平研究和临床试验。
