Wen Xiao, Guo Xing-yi, Fan Long-jiang
Institute of Crop Science, Zhejiang University, Hangzhou 310029, China.
J Zhejiang Univ Sci B. 2005 Jun;6(6):470-6. doi: 10.1631/jzus.2005.B0470.
Mutation (substitution, deletion, insertion, etc.) in nucleotide acid causes the maximal sequence lengths of exact match (MALE) between paralogous members from a duplicate event to become shorter during evolution. In this work, MALE changes between members of 26 gene families from four representative species (Arabidopsis thaliana, Oryza sativa, Mus musculus and Homo sapiens) were investigated. Comparative study of paralogous' MALE and amino acid substitution rate (d(A)<0.5) indicated that a close relationship existed between them. The results suggested that MALE could be a sound evolutionary scale for the divergent time for paralogous genes during their early evolution. A reference table between MALE and divergent time for the four species was set up, which would be useful widely, for large-scale genome alignment and comparison. As an example, detection of large-scale duplication events of rice genome based on the table was illustrated.
核酸中的突变（替换、缺失、插入等）会导致重复事件中旁系同源成员之间的最大精确匹配序列长度（MALE）在进化过程中变短。在这项研究中，我们调查了来自四个代表性物种（拟南芥、水稻、小家鼠和智人）的26个基因家族成员之间的MALE变化。对旁系同源物的MALE和氨基酸替换率（d(A)<0.5）的比较研究表明，它们之间存在密切关系。结果表明，MALE可能是旁系同源基因早期进化过程中分歧时间的一个可靠进化尺度。我们建立了这四个物种的MALE与分歧时间之间的参考表，这将广泛应用于大规模基因组比对和比较。作为一个例子，我们说明了基于该表检测水稻基因组的大规模重复事件。
