利用响应面法优化米渣水解物对血管紧张素I转换酶（ACE）的抑制作用
Optimization of angiotensin I-converting enzyme (ACE) inhibition by rice dregs hydrolysates using response surface methodology.
作者信息
He Guo-qing, Xuan Guo-dong, Ruan Hui, Chen Qi-he, Xu Ying
机构信息
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China.
出版信息
J Zhejiang Univ Sci B. 2005 Jun;6(6):508-13. doi: 10.1631/jzus.2005.B0508.
Angiotensin I-converting enzyme (ACE) inhibitory peptides have been shown to have antihypertensive effects and have been utilized for physiologically functional foods and pharmaceuticals. The ACE inhibitory ability of a hydrolysate is determined by its peptide composition. However, the peptide composition of a hydrolysate depends on proteolytic enzyme and the hydrolysis conditions. In this study, the effect of process conditions on the ACE inhibitory activity of rice dregs hydrolyzed with a trypsin was investigated systematically using response surface methodology. It was shown that the ACE inhibitory activity of rice dregs hydrolysates could be controlled by regulation of five process conditions. Hydrolysis conditions for optimal ACE inhibition were defined using the response surface model of fractional factorial design (FFD), steepest ascent design, and central composite design (CCD).
血管紧张素I转换酶（ACE）抑制肽已被证明具有降压作用，并已被用于生理功能食品和药品中。水解产物的ACE抑制能力由其肽组成决定。然而，水解产物的肽组成取决于蛋白酶和水解条件。在本研究中，使用响应面法系统地研究了工艺条件对用胰蛋白酶水解的米渣ACE抑制活性的影响。结果表明，米渣水解产物的ACE抑制活性可通过调节五个工艺条件来控制。使用分数析因设计（FFD）、最速上升设计和中心复合设计（CCD）的响应面模型确定了最佳ACE抑制的水解条件。
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