在液相色谱/质谱联用或气相色谱/质谱联用之前，通过二维液相色谱进行碳水化合物预纯化的新型、高度稳健的方法。
Novel, highly robust method of carbohydrate pre-purification by two-dimensional liquid chromatography prior to liquid chromatography/mass spectrometry or gas chromatography/mass spectrometry.
作者信息
Rogatsky Eduard, Stein Daniel
机构信息
Albert Einstein College of Medicine of Yeshiva University, General Clinical Research Center Analytical Core Laboratory, 1300 Morris Park Avenue, New York, Room G47, Bronx, NY 10461, USA.
出版信息
J Chromatogr A. 2005 May 6;1073(1-2):11-6. doi: 10.1016/j.chroma.2005.03.074.
We describe a novel two-dimensional liquid chromatography (2D-LC) method for fast and robust isolation and concentration of low abundant carbohydrates (sorbitol, glycerol) from biological matrices (plasma and urine). Off-line pre-purified fractions, enriched by analyte of interest, were analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS-MS). Initial 2D-LC automated sample pre-purification improved MS detection, eliminated matrix effects, and achieved high sensitivity (picogram detection limit) with a 6 min runtime and increased column lifetime. Using this method we have analyzed more than 1300 samples from biological matrices without column replacement.
我们描述了一种新型二维液相色谱（2D-LC）方法，用于从生物基质（血浆和尿液）中快速、稳健地分离和浓缩低丰度碳水化合物（山梨醇、甘油）。通过感兴趣的分析物富集的离线预纯化馏分，采用液相色谱/电喷雾电离串联质谱（LC/ESI-MS-MS）进行分析。最初的二维液相色谱自动样品预纯化提高了质谱检测能力，消除了基质效应，并在6分钟的运行时间内实现了高灵敏度（皮克检测限），同时延长了色谱柱寿命。使用该方法，我们在不更换色谱柱的情况下分析了来自生物基质的1300多个样品。
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