Buravkova L B, Rykova M P, Antropova E N, Grigor'eva O V
Aviakosm Ekolog Med. 2005 Jan-Feb;39(1):55-9.
A space modification of a method for determining the cytotoxic activity of natural killers (NK) uses cultures of human lymphocytes and re-inoculated suspension of tumorous myeloblasts K-562. The 3H-uridine labeled target-cells fixated in 0.1 % formalin solution proved to be best suited to the conditions prohibiting regular recharge of cultural medium and utilization of radioactive agents. The following practical conclusions were made from the space-flown experiment: pre-launch procedures (lymphocyte isolation and delivery to the launch site) and time period till dock must not be longer than 5 days (1) and during transportation to the orbiting vehicle cell cultures must be kept at 18-20 degrees C (2). The modified method can be employed to study various aspects of cell interaction such as the NK ability to destroy target-cells in suspension cultured exposed in microgravity.
一种用于测定自然杀伤细胞（NK）细胞毒性活性方法的空间改进，使用人淋巴细胞培养物和重新接种的肿瘤性成髓细胞K - 562悬浮液。经证明，固定在0.1%福尔马林溶液中的3H - 尿苷标记靶细胞最适合禁止定期更换培养基和使用放射性试剂的条件。从太空飞行实验得出以下实际结论：发射前程序（淋巴细胞分离并运送到发射地点）和直到对接的时间段不得超过5天（1），并且在运送到轨道飞行器的过程中，细胞培养物必须保持在18 - 20摄氏度（2）。改进后的方法可用于研究细胞相互作用的各个方面，例如自然杀伤细胞在微重力下悬浮培养中破坏靶细胞的能力。
