Aurer Andrej, Stavljenić-Rukavina Ana, Aurer-Kozelj Jelena
Zavod za parodonltologiju Stomatoloskog fakulteta Sveucilista u Zagrebu, Hrvatska, Croatia.
Acta Med Croatica. 2005;59(2):117-22.
Periodontal diseases result from interactions between periodontal microflora and the multifaceted response of the host. Markers of this interrelationship can be found in gingival crevicular fluid and saliva samples indicating early periodontal disease activity. Studies of host defense mediators in saliva of patients with periodontal diseases are very scarce.
The goal of this study was therefore to investigate interrelationships between mediators of local immunity in host saliva and clinical parameters of periodontal destruction in persons with chronic and aggressive periodontitis.
Host defense indicators were measured using enzyme-immunochemistry tests in unstimulated saliva samples of persons with periodontitis. The degree of periodontal destruction was determined by measuring attachment loss. Subjects were divided into two groups: persons with mild signs of chronic periodontitis and persons with severe signs of aggressive periodontitis. Hypotheses about the structure of those samples and their interrelationship were tested using non-parametric methods.
Results indicated that C-reactive protein, C3 component of the complement, alfa 2-macroglobulin and interleukin-6 levels differ significantly between patients with chronic and aggressive periodontitis. Interleukin-6, influencing cells with osteoblastic phenotype, is present at a high level in patients with aggressive periodontitis. Calcium was much lower than in healthy persons. Trypsin levels were lower in patients with periodontitis than in healthy persons. We found a significant interrelationship between host response mediators as measured in saliva and periodontal destruction measured by clinical attachment loss.
The levels of host defense mediators in saliva are different for chronic and aggressive periodontitis. This findings might help in estimating the course and progression of periodontal destruction, thus providing clinical benefits in treatment planning.
牙周疾病是由牙周微生物群与宿主的多方面反应相互作用所致。这种相互关系的标志物可在龈沟液和唾液样本中找到，表明早期牙周疾病活动。关于牙周疾病患者唾液中宿主防御介质的研究非常匮乏。
因此，本研究的目的是调查慢性和侵袭性牙周炎患者宿主唾液中局部免疫介质与牙周破坏临床参数之间的相互关系。
使用酶免疫化学检测法测量牙周炎患者未刺激唾液样本中的宿主防御指标。通过测量附着丧失来确定牙周破坏程度。受试者分为两组：慢性牙周炎轻度体征患者和侵袭性牙周炎重度体征患者。使用非参数方法检验关于这些样本结构及其相互关系的假设。
结果表明，慢性和侵袭性牙周炎患者之间的C反应蛋白、补体C3成分、α2巨球蛋白和白细胞介素-6水平存在显著差异。影响具有成骨细胞表型细胞的白细胞介素-6在侵袭性牙周炎患者中高水平存在。钙含量远低于健康人。牙周炎患者的胰蛋白酶水平低于健康人。我们发现唾液中测量的宿主反应介质与临床附着丧失测量的牙周破坏之间存在显著的相互关系。
慢性和侵袭性牙周炎患者唾液中宿主防御介质水平不同。这一发现可能有助于评估牙周破坏的进程，从而在治疗计划中提供临床益处。
