脂质过氧化：在慢性牙周炎的诱发和进展中可能发挥的作用。

Lipid peroxidation: a possible role in the induction and progression of chronic periodontitis.

作者信息

Tsai C C, Chen H S, Chen S L, Ho Y P, Ho K Y, Wu Y M, Hung C C

机构信息

Graduate Institute of Dental Sciences, College of Dental Medicine, Kaohsiung Medical University, Taiwan.

出版信息

J Periodontal Res. 2005 Oct;40(5):378-84. doi: 10.1111/j.1600-0765.2005.00818.x.

DOI:10.1111/j.1600-0765.2005.00818.x

PMID:16105090

Abstract

OBJECTIVES

Reactive oxygen species (ROS) are implicated in the destruction of the periodontium during inflammatory periodontal diseases. The imbalance in oxidant/antioxidant activity may be a key factor in the damaging effects of ROS. This study aimed to determine the lipid peroxidation levels in gingival crevicular fluid and saliva, and glutathione (GSH) and glutathione peroxidase (GPx) in saliva in patients with chronic periodontitis.

METHODS

Gingival crevicular fluid and saliva were collected from 13 patients and 9 healthy control subjects during the preliminary study, and from 21 patients during the subsequent study. Lipid peroxidation level, GSH level and GPx activity were determined by spectrophotometric assay.

RESULTS

The preliminary study found that when comparing patients to healthy controls, the gingival crevicular fluid samples produced the following results, respectively: higher lipid peroxidation concentration (microm) (by sites: 167.55 vs. 53.71, p < 0.0001; by subjects: 151.99 vs. 50.66, p < 0.005) and total amount (pmol) (by sites: 93.02 vs. 8.47, p < 0.0001, by subjects: 80.44 vs. 7.84, p < 0.0005). In saliva samples, lower GSH concentration (microm) (373.04 vs. 606.67, p < 0.05), higher lipid peroxidation concentration (microm) (0.66 vs. 0.13, p < 0.0005), and no difference in GPx activity were found in patients than in those of healthy controls. The subsequent study showed statistically significant (p < 0.05) improvement of clinical periodontal parameters (plaque index, gingival index, probing attachment level, probing pocket depth and gingival crevicular fluid volume), decreases in gingival crevicular fluid lipid peroxidation levels (concentration and total amount) at the sites after the completion of phase 1 periodontal treatment. Similarly, the periodontal treatment resulted in a significant decrease of lipid peroxidation concentrations (p < 0.05), increase in GSH concentration (p < 0.001), and no change in GPx activity in saliva samples.

CONCLUSION

The increased levels of lipid peroxidation may play a role in the inflammation and destruction of the periodontium in periodontitis.

摘要

目的

活性氧（ROS）与炎症性牙周疾病期间牙周组织的破坏有关。氧化/抗氧化活性失衡可能是ROS产生破坏作用的关键因素。本研究旨在测定慢性牙周炎患者龈沟液和唾液中的脂质过氧化水平，以及唾液中的谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx）。

方法

在初步研究中，从13例患者和9名健康对照者中采集龈沟液和唾液，在后续研究中从21例患者中采集。通过分光光度法测定脂质过氧化水平、GSH水平和GPx活性。

结果

初步研究发现，与健康对照相比，龈沟液样本分别产生以下结果：脂质过氧化浓度（微摩尔）更高（按部位：167.55对53.71，p<0.0001；按个体：151.99对50.66，p<0.005）和总量（皮摩尔）更高（按部位：93.02对8.47，p<0.0001，按个体：80.44对7.84，p<0.0005）。在唾液样本中，患者的GSH浓度（微摩尔）较低（373.04对606.67，p<0.05），脂质过氧化浓度（微摩尔）较高（0.66对0.13，p<0.0005），且GPx活性与健康对照者无差异。后续研究显示，在完成第一阶段牙周治疗后，临床牙周参数（菌斑指数、牙龈指数、探诊附着水平、探诊袋深度和龈沟液量）有统计学意义的改善（p<0.05），龈沟液脂质过氧化水平（浓度和总量）在各部位均下降。同样，牙周治疗导致唾液样本中脂质过氧化浓度显著降低（p<0.05），GSH浓度升高（p<0.001），GPx活性无变化。