Tobón-Arroyave S I, Jaramillo-González P E, Isaza-Guzmán D M
POPCAD Research Group, Laboratory of Immunodetection and Bioanalysis, Faculty of Dentistry, University of Antioquia, Medellín, Colombia.
Arch Oral Biol. 2008 Apr;53(4):346-52. doi: 10.1016/j.archoralbio.2007.11.005. Epub 2007 Dec 21.
To assess the concentration of the proinflammatory cytokine IL-1beta in saliva of periodontally diseased and healthy patients and their relationship with the periodontal status.
Unstimulated whole saliva samples from patients with chronic periodontitis (n=30), aggressive periodontitis (n=18) and healthy controls (n=18) were obtained for the study. The periodontal status of each subject was assessed by criteria based on probing depth, clinical attachment loss and the extent/severity of periodontal breakdown. The levels of IL-1beta were measured in saliva samples with a high sensitivity enzyme-linked immunosorbent assay (ELISA).
Although no significant difference (P=0.624) was found for salivary IL-1beta levels between periodontitis groups, they were significantly greater (P<0.01) than those detected for healthy controls. Furthermore, Spearman correlation analysis showed statistically significant correlations (P<0.01) between data from salivary IL-1beta levels and clinical measurements.
The findings of the present study reemphasize the importance of whole saliva as sampling method in terms of immunological purposes in periodontal disease and suggest that the elevated IL-1beta concentration may be one of the host-response components associated to the clinical manifestations of periodontal disease.
评估牙周病患者和健康患者唾液中促炎细胞因子白细胞介素-1β（IL-1β）的浓度及其与牙周状况的关系。
采集慢性牙周炎患者（n = 30）、侵袭性牙周炎患者（n = 18）和健康对照者（n = 18）的非刺激性全唾液样本进行研究。根据探诊深度、临床附着丧失以及牙周破坏的范围/严重程度等标准评估每个受试者的牙周状况。采用高灵敏度酶联免疫吸附测定（ELISA）法检测唾液样本中IL-1β的水平。
虽然牙周炎组之间唾液IL-1β水平未发现显著差异（P = 0.624），但与健康对照者相比，其水平显著更高（P < 0.01）。此外，Spearman相关性分析显示，唾液IL-1β水平数据与临床测量数据之间存在统计学显著相关性（P < 0.01）。
本研究结果再次强调了全唾液作为牙周病免疫研究采样方法的重要性，并表明IL-1β浓度升高可能是与牙周病临床表现相关的宿主反应成分之一。
