Su Rui Jun, Li Karen, Zhang Xiao Bing, Pan Yuen Patrick Man, Li Chi Kong, James Anthony Edward, Liu Jie, Fok Tai Fai
Department of Paediatrics, The Chinese University of Hong Kong, Hong Kong.
Stem Cells Dev. 2005 Apr;14(2):223-30. doi: 10.1089/scd.2005.14.223.
Stem cell expansion remains an elusive but highly desirable goal. Here we show that platelet-derived growth factor (PDGF), along with cultured endothelial or stromal cells, significantly enhances expansion of human CD34+ cells in vitro. In media supplemented with thrombopoietin, stem cell factor, flt-3 ligand, and granulocyte-colony stimulating factor, CD34+ cells, as well as CFU-GM, BFU-CFU-E, CFU-GEMM, and CFU-MK, increased by 34.3-, 138-, 59.7-, 38.4-, and 86.0-fold, respectively. Co-culturing of CD34+ cells with cultured stromal cells or human umbilical cord vein endothelial cells (HUVECs) greatly enhanced expansion efficiency. The presence of PDGF (50 ng/ml) further augmented expansion, such that increases of 77.0-, 262-, 90.0-, 93.0-, and 200-fold, respectively, were achieved. Six weeks after infusion of expanded cells into NOD/SCID mice, human CD45+ cells were detected in recipients' bone marrow, spleen, and peripheral blood. Our results provide a rationale for development of a stem cell expansion protocol for clinical applications.
干细胞扩增仍然是一个难以实现但非常令人向往的目标。在此我们表明，血小板衍生生长因子（PDGF）与培养的内皮细胞或基质细胞一起，能在体外显著增强人CD34+细胞的扩增。在补充了血小板生成素、干细胞因子、fms样酪氨酸激酶3配体和粒细胞集落刺激因子的培养基中，CD34+细胞以及粒系巨噬系集落形成单位（CFU-GM）、爆式红系集落形成单位（BFU-CFU-E）、粒-红-巨噬-巨核系集落形成单位（CFU-GEMM）和巨核系集落形成单位（CFU-MK）分别增加了34.3倍、138倍、59.7倍、38.4倍和86.0倍。将CD34+细胞与培养的基质细胞或人脐静脉内皮细胞（HUVECs）共培养可大大提高扩增效率。PDGF（50 ng/ml）的存在进一步增强了扩增效果，分别实现了77.0倍、262倍、90.0倍、93.0倍和200倍的增加。将扩增后的细胞输注到NOD/SCID小鼠体内六周后，在受体的骨髓、脾脏和外周血中检测到了人CD45+细胞。我们的结果为开发用于临床应用的干细胞扩增方案提供了理论依据。
