Riesz Jennifer, Gilmore Joel, Meredith Paul
Soft Condensed Matter Physics Group and Centre for Biophotonics and Laser Science, Department of Physics, University of Queensland, St. Lucia, Brisbane, Qld 4072, Australia.
Spectrochim Acta A Mol Biomol Spectrosc. 2005 Jul;61(9):2153-60. doi: 10.1016/j.saa.2004.08.014.
We report methods for correcting the photoluminescence emission and excitation spectra of highly absorbing samples for re-absorption and inner filter effects. We derive the general form of the correction, and investigate various methods for determining the parameters. Additionally, the correction methods are tested with highly absorbing fluorescein and melanin (broadband absorption) solutions; the expected linear relationships between absorption and emission are recovered upon application of the correction, indicating that the methods are valid. These procedures allow accurate quantitative analysis of the emission of low quantum yield samples (such as melanin) at concentrations where absorption is significant.
我们报告了用于校正高吸收性样品的光致发光发射光谱和激发光谱以消除再吸收和内滤效应的方法。我们推导了校正的一般形式，并研究了确定参数的各种方法。此外，用高吸收性的荧光素和黑色素（宽带吸收）溶液对校正方法进行了测试；应用校正后恢复了吸收与发射之间预期的线性关系，表明这些方法是有效的。这些程序能够对吸收显著的浓度下低量子产率样品（如黑色素）的发射进行准确的定量分析。
