基因表达谱分析以表征抗癌药物敏感性。

Gene expression profiling to characterize anticancer drug sensitivity.

作者信息

Breaux James K, Los Gerrit

机构信息

Rebecca and John Moores UCSD Cancer Center, University of California-San Diego, La Jolla, USA.

出版信息

Methods Mol Med. 2005;111:197-231. doi: 10.1385/1-59259-889-7:197.

DOI:10.1385/1-59259-889-7:197

PMID:15911981

Abstract

This chapter presents a protocol for using cDNA microarrays to acquire gene expression profiles that characterize anticancer drug sensitivity. The protocol includes steps for drug exposure, RNA isolation, preparation of fluorescently labeled samples, microarray hybridization, data processing, and data analysis. In addition to the detailed protocol, important experimental design issues are discussed, and some preliminary experiments are recommended.

摘要

本章介绍了一种使用cDNA微阵列获取表征抗癌药物敏感性的基因表达谱的方案。该方案包括药物暴露、RNA分离、荧光标记样品制备、微阵列杂交、数据处理和数据分析等步骤。除了详细的方案外，还讨论了重要的实验设计问题，并推荐了一些初步实验。