Tan Feng, Yang Bingcheng, Guan Yafeng
Department of Micro-Instrument and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Anal Sci. 2005 May;21(5):583-5. doi: 10.2116/analsci.21.583.
A combined detection system of simultaneous contactless conductometric and fluorescent detection for capillary electrophoresis (CE) has been designed and evaluated. The two processes share a common detection cell. A blue light-emitting diode (LED) was used as the excitation source and an optical fiber was used to collect the emitting fluorescence for fluorescent detection (FD). Inorganic ions, fluorescein isothiocyanate (FITC)-labeled amino acids and small molecule peptides were separated and detected by the combined detector, and the detection limits (LODs) of sub-microM level were achieved.
设计并评估了一种用于毛细管电泳（CE）的同时非接触式电导和荧光检测的组合检测系统。这两个过程共享一个通用检测池。使用蓝色发光二极管（LED）作为激发源，并使用光纤收集发射的荧光以进行荧光检测（FD）。通过该组合检测器对无机离子、异硫氰酸荧光素（FITC）标记的氨基酸和小分子肽进行了分离和检测，实现了亚微摩尔级的检测限。
