Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, P.O. Box 100, Dalian 116012, People's Republic of China.
Talanta. 2003 Mar 1;59(3):509-14. doi: 10.1016/s0039-9140(02)00531-3.
A simple fluorescence detector for capillary electrophoresis (CE) using a blue light-emitting-diode (LED) as excitation source is constructed and evaluated. An optical fiber was used to collect the fluorescence, and a flat end of the fiber was modified to spherical end, resulting in 50% increase of efficiency over the flat end. A simple device for optical alignment of the fibers and capillary column was designed. The concentration and mass detection limits for fluorescein were 1.8x10(-7) mol l(-1) and 4.3 femol, respectively.
构建并评估了一种使用蓝光发光二极管（LED）作为激发光源的毛细管电泳（CE）简易荧光检测器。使用光纤收集荧光，将光纤的平头改为半球形端，效率提高了 50%。设计了一种用于光纤和毛细管柱光学对准的简单装置。荧光素的浓度和质量检测限分别为 1.8x10(-7) mol l(-1) 和 4.3 femol。
