Dowd Patrick F, Johnson Eric T
National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, USA.
J Agric Food Chem. 2005 Jun 1;53(11):4464-70. doi: 10.1021/jf0404750.
The presence of a pI 9.0 cationic peroxidase isozyme from milk stage pericarp of six susceptible and five resistant inbreds was correlated significantly with previously reported field data on percentage infection by Aspergillus flavus in the inbreds and their hybrids. The isozyme was constitutively expressed in some additional maize tissues and lines examined, and frequently induced by mechanical damage, heat shock, Fusarium proliferatum, and/or Bacillus subtilis in other lines tested. Native/IEF two-dimensional electrophoresis identified the isozyme as the previously genetically identified px5. A cDNA clone expressed in black Mexican sweet (BMS) maize cell cultures produced the pI 9.0 isozyme. In addition to potential use in marker-assisted breeding, enhanced expression of this cationic peroxidase through breeding or genetic engineering may lead to enhanced disease or insect resistance.
来自六个感病自交系和五个抗病自交系乳熟期果皮的一种等电点为9.0的阳离子过氧化物酶同工酶的存在，与之前报道的关于这些自交系及其杂交种中黄曲霉感染百分比的田间数据显著相关。该同工酶在其他检测的玉米组织和品系中组成性表达，并且在其他测试品系中经常受到机械损伤、热激、层出镰刀菌和/或枯草芽孢杆菌的诱导。天然/等电聚焦二维电泳将该同工酶鉴定为之前通过遗传学方法鉴定的px5。在黑色墨西哥甜玉米（BMS）细胞培养物中表达的一个cDNA克隆产生了等电点为9.0的同工酶。除了在标记辅助育种中的潜在用途外，通过育种或基因工程增强这种阳离子过氧化物酶的表达可能会增强对病害或虫害的抗性。
