Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, Harbin, China.
Mol Biol Rep. 2010 Mar;37(3):1183-90. doi: 10.1007/s11033-009-9484-8. Epub 2009 Mar 1.
The ThPOD1 gene encodes a peroxidase and was isolated from a Tamarix hispida NaCl-stress root cDNA library. We found that ThPOD1 expression could be induced by abiotic stresses such as cold, salt, drought and exogenous abscisic acid. These findings suggested that ThPOD1 might be involved in the plant response to environmental stresses and ABA treatment. To elucidate the function of this gene, recombinant plasmids expressing full-length ThPOD1 as well as ThPOD2 (aa 41-337), and ThPOD3 (aa 73-337) truncated polypeptides were constructed. SDS-PAGE and Western blot analyses of the fusion proteins revealed that the molecular weights of ThPOD1, ThPOD2 and ThPOD3 were approximately 57, approximately 50 and approximately 47 kDa, respectively. Stress assays of E. coli treated with the recombinant plasmids indicated that ThPOD3 could improve resistance to drought stress. This finding could potentially be used to improve plant tolerance to drought stress via gene transfer.
ThPOD1 基因编码一种过氧化物酶，它是从柽柳 NaCl 胁迫根 cDNA 文库中分离得到的。我们发现，ThPOD1 的表达可以被非生物胁迫诱导，如冷、盐、干旱和外源脱落酸。这些发现表明，ThPOD1 可能参与植物对环境胁迫和 ABA 处理的反应。为了阐明该基因的功能，构建了表达全长 ThPOD1 以及 ThPOD2（aa41-337）和 ThPOD3（aa73-337）截断多肽的重组质粒。融合蛋白的 SDS-PAGE 和 Western blot 分析表明，ThPOD1、ThPOD2 和 ThPOD3 的分子量约为 57、50 和 47 kDa。用重组质粒处理大肠杆菌的应激试验表明，ThPOD3 可以提高对干旱胁迫的抗性。这一发现可能通过基因转移来提高植物对干旱胁迫的耐受性。
