Weitlauf H M, Knisley K A
Department of Cell Biology, Texas Tech University Health Sciences Center, Lubbock 79430.
Biol Reprod. 1992 May;46(5):811-6. doi: 10.1095/biolreprod46.5.811.
Changes in the expression of specific cell surface antigens on preimplantation mouse embryos were examined by immunocytochemistry. Embryos were recovered at various times during the preimplantation phase of normal pregnancy, and from pregnancies with experimentally induced delayed implantation, and were probed with a panel of monoclonal antibodies against murine leukocyte antigens. Antibodies directed against certain macrophage surface glycoproteins (i.e., Mac-2 and Mac-3) and those against lysosome-associated membrane glycoproteins (i.e., LAMP-1 and LAMP-2) reacted specifically with cell surface determinants on the embryos. Differences in the spatiotemporal patterns of antibody binding during normal and delayed implantation indicate that expression of the antigenic determinants recognized by these antibodies is regulated individually in response to intrinsic as well as extrinsic signals at the time of implantation, and thus they may be important for the process of embryo implantation.
通过免疫细胞化学方法检测了植入前小鼠胚胎上特定细胞表面抗原的表达变化。在正常妊娠植入前期的不同时间，以及实验诱导延迟着床的妊娠中回收胚胎，并用一组针对鼠白细胞抗原的单克隆抗体进行检测。针对某些巨噬细胞表面糖蛋白（即Mac-2和Mac-3）的抗体以及针对溶酶体相关膜糖蛋白（即LAMP-1和LAMP-2）的抗体与胚胎上的细胞表面决定簇发生特异性反应。正常着床和延迟着床期间抗体结合的时空模式差异表明，这些抗体识别的抗原决定簇的表达在着床时会根据内在和外在信号分别进行调节，因此它们可能对胚胎着床过程很重要。
