Knisley K A, Weitlauf H M
Department of Cell Biology, Texas Tech University Health Sciences Center, Lubbock 79430.
J Reprod Fertil. 1993 Mar;97(2):521-7. doi: 10.1530/jrf.0.0970521.
Changes in the expression of antigens on mouse uterine or embryonic tissues were examined by enzyme immunocytochemistry. Cryostat sections of uteri from days 1, 8 and 15 of pregnancy were probed with the monoclonal antibodies M3/38 and M3/84, originally defined by their reactivity with macrophage surface antigens (Mac-2 and Mac-3, respectively). In the uterus of pregnant mice, reaction of these antibodies was not limited to leucocytes. M3/38 did not react at detectable levels with cells in the uterus on day 1 but did react with decidual cells immediately surrounding the embryo on day 8. By day 15, the placenta, decidua basalis and metrial gland were intensely positive but the embryo was negative. M3/84 reacted with the luminal side of the endometrium on day 1, the entire decidual mass on day 8, and with all maternal and fetal tissues on day 15. M3/38 was detected in saline-soluble preparations of uterine tissue and had a molecular mass of approximately 32-35 kDa as determined by SDS-PAGE and western blot analysis. The pattern of expression of these molecules suggests a functional relationship to developmental changes that occur at the maternal-fetal interface.
通过酶免疫细胞化学法检测小鼠子宫或胚胎组织上抗原表达的变化。用单克隆抗体M3/38和M3/84探测妊娠第1天、第8天和第15天子宫的冰冻切片，这两种抗体最初分别根据它们与巨噬细胞表面抗原（分别为Mac-2和Mac-3）的反应性来定义。在妊娠小鼠的子宫中，这些抗体的反应并不局限于白细胞。M3/38在第1天与子宫中的细胞无明显可检测水平的反应，但在第8天与紧邻胚胎的蜕膜细胞有反应。到第15天，胎盘、基底蜕膜和子宫肌腺呈强阳性，但胚胎为阴性。M3/84在第1天与子宫内膜的腔面有反应，在第8天与整个蜕膜团有反应，在第15天与所有母体和胎儿组织有反应。在子宫组织的盐溶性制剂中检测到M3/38，通过SDS-PAGE和蛋白质免疫印迹分析确定其分子量约为32 - 35 kDa。这些分子的表达模式表明它们与母胎界面发生的发育变化存在功能关系。
