Yang Qing-Xiong, Zhang Ying-Jun, Li Hai-Zhou, Yang Chong-Ren
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
Steroids. 2005 Sep;70(10):732-7. doi: 10.1016/j.steroids.2005.04.003.
Six new polyhydroxylated steroidal saponins, tupistrosides A-F (1-6), together with nine known steroids, were isolated from the fresh rhizomes of Tupistra yunnanensis. Their structures were elucidated to be (25S)-1beta,4beta,5beta-trihydroxy-spirostane-3beta-yl O-alpha-l-arabinopyranoside (1), 1beta,24beta-dihydroxy-spirost-5,25(27)-dien-3alpha-yl O-beta-d-glucopyranoside (2), (22S,25S)-1alpha,2beta,3alpha,5alpha-tetrahydroxy-furo-spirostane-26-yl O-beta-d-glucopyranoside (3), 1beta,3alpha,22 xi-trihydroxy-furost-5,25(27)-dien-26-yl O-beta-d-glucopyranoside (4), 26-O-beta-d-glucopyranosyl-1beta,22-dihydroxy-furost-5-en-3alpha-yl O-beta-d-glucopyranoside (5) and 22-methoxy-1beta,2beta,3beta,4beta,5beta,7alpha-hexahydroxy-furost-25(27)-en-6-one-26-yl O-beta-d-glucopyranoside (6), respectively, by means of spectroscopic analysis and the results of acid hydrolysis.
从滇开口箭的新鲜根茎中分离得到6个新的多羟基甾体皂苷，开口箭皂苷A - F（1 - 6），以及9个已知甾体化合物。通过光谱分析和酸水解结果，确定它们的结构分别为(25S)-1β,4β,5β-三羟基螺旋甾烷-3β-基O-α-L-阿拉伯吡喃糖苷（1）、1β,24β-二羟基螺旋甾-5,25(27)-二烯-3α-基O-β-D-葡萄糖吡喃糖苷（2）、(22S,25S)-1α,2β,3α,5α-四羟基呋甾烷-26-基O-β-D-葡萄糖吡喃糖苷（3）、1β,3α,22ξ-三羟基呋甾-5,25(27)-二烯-26-基O-β-D-葡萄糖吡喃糖苷（4）、26-O-β-D-葡萄糖吡喃糖基-1β,22-二羟基呋甾-5-烯-3α-基O-β-D-葡萄糖吡喃糖苷（5）和22-甲氧基-1β,2β,3β,4β,
