Uttayamakul Sumonmal, Likanonsakul Sirirat, Sunthornkachit Rujanee, Kuntiranont Karoon, Louisirirotchanakul Suda, Chaovavanich Achara, Thiamchai Vipa, Tanprasertsuk Sombat, Sutthent Ruengpung
National HIV Repository and Bioinformatic Center, Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkok 10700, Thailand.
J Virol Methods. 2005 Sep;128(1-2):128-34. doi: 10.1016/j.jviromet.2005.04.010.
The usage of dried blood spots as specimens for diagnosis and monitoring of HIV-1 infection in Thailand was evaluated. EDTA blood samples, which were collected from 100 HIV seronegative and 109 HIV seropositive individuals, were tested on dried blood spots; Whatman, Schleicher and Schuell (S&S) No. 903 and S&S IsoCode filter paper. Nucleic acid was extracted and used as a template for HIV-1 proviral DNA detection by an "in-house" multiplex PCR and a commercial Amplicor HIV-1 PCR test (Roche, version 1.0). HIV-1 RNA qualitative (QL) and quantitative (QT) detection was determined by Nucleic Acid Sequence Based Amplification (NASBA). The average DNA per blood spot recovered from Whatman and S&S IsoCode was not statistically different (p = 0.512) with a range of 218.9+/-46.84 and 225.63+/-88.33 microg, respectively. The concordance of HIV-1 proviral DNA detection by PCR from dried blood spots Whatman and S&S IsoCode was 94% versus 89.4% for sensitivity and 100% versus 100% for specificity. The sensitivity and specificity of HIV-1 RNA QL detection in dried blood spots was 89.7 and 97.5%, respectively. The HIV-1 RNA QT from dried blood spots showed a good correlation in paired dried blood spots and plasma with Pearson correlation, r = 0.817 (R2 = 0.667, P < 0.05). The data showed that dried blood spots could be used for the diagnosis and monitoring of HIV-1 infection.
对在泰国将干血斑用作人类免疫缺陷病毒1型（HIV-1）感染诊断和监测样本的用途进行了评估。从100名HIV血清阴性个体和109名HIV血清阳性个体采集的乙二胺四乙酸（EDTA）血样，在干血斑上进行检测；沃特曼、施莱歇尔和舒尔（S&S）903号滤纸以及S&S IsoCode滤纸。提取核酸，并将其用作通过“内部”多重聚合酶链反应（PCR）和商业的Amplicor HIV-1 PCR检测（罗氏，1.0版）检测HIV-1前病毒DNA的模板。通过基于核酸序列的扩增技术（NASBA）进行HIV-1 RNA定性（QL）和定量（QT）检测。从沃特曼滤纸和S&S IsoCode滤纸回收的每个血斑的平均DNA无统计学差异（p = 0.512），范围分别为218.9±46.84和225.63±88.33微克。通过PCR检测干血斑沃特曼滤纸和S&S IsoCode滤纸上HIV-1前病毒DNA的一致性，敏感性分别为94%和89.4%，特异性分别为100%和100%。干血斑中HIV-1 RNA QL检测的敏感性和特异性分别为89.7%和97.5%。干血斑中的HIV-1 RNA QT在配对的干血斑和血浆中显示出良好的相关性，皮尔逊相关系数r = 0.817（R2 = 0.667，P < 0.05）。数据表明，干血斑可用于HIV-
