Yoshihara N, Fukushima K, Suzuki H, Honda M, Yamazaki S, Fukutake K, Meguro T, Yamada K
AIDS Research Center, National Institute of Health, Shinjuku-ku, Tokyo.
Rinsho Byori. 1993 Dec;41(12):1323-7.
AMPLICOR HIV-1 test kits, which had been developed as an HIV-1 provirus detection test by PCR method, have been evaluated for its clinical diagnostic application. Sixty-six of HIV-1 antibody positive and 67 of HIV-1 antibody negative blood samples derived from hemophiliacs, who had received blood products, have been tested by AMPLICOR HIV-1. All of the results from AMPLICOR HIV-1 were consistent with those from antibody test and clinical aspects. Thirty-nine of HIV-1 antibody positive samples have been tested by AMPLICOR HIV-1 and virus isolation (culture method). Twelve of 39 (30.8%) were positive by virus isolation, and 39 of 39 (100%) were positive by AMPLICOR HIV-1. Two of new born infants from HIV-1 sero-positive mothers were tested by AMPLICOR HIV-1, and the result suggested that the kit would be useful for diagnosis of infants from sero-positive mothers. Based on these studies, AMPLICOR HIV-1 is considered as useful clinical diagnostic for HIV-1 proviral DNA detection.
AMPLICOR HIV-1检测试剂盒是通过聚合酶链反应（PCR）方法开发的用于检测HIV-1前病毒的检测试剂盒，已对其临床诊断应用进行了评估。对66份来自接受过血液制品的血友病患者的HIV-1抗体阳性血样和67份HIV-1抗体阴性血样进行了AMPLICOR HIV-1检测。AMPLICOR HIV-1的所有检测结果均与抗体检测结果及临床情况一致。对39份HIV-1抗体阳性样本进行了AMPLICOR HIV-1检测和病毒分离（培养法）。39份样本中12份（30.8%）病毒分离呈阳性，39份（100%）AMPLICOR HIV-1检测呈阳性。对2名来自HIV-1血清学阳性母亲的新生儿进行了AMPLICOR HIV-1检测，结果表明该试剂盒对诊断血清学阳性母亲的婴儿有用。基于这些研究，AMPLICOR HIV-1被认为是检测HIV-1前病毒DNA的有用临床诊断方法。
