Pleasance S, Ayer S W, Laycock M V, Thibault P
Marine Analytical Chemistry Standards Program, National Research Council of Canada, Halifax, Nova Scotia.
Rapid Commun Mass Spectrom. 1992 Jan;6(1):14-24. doi: 10.1002/rcm.1290060105.
Ionspray mass spectrometry has been used to monitor the purification of saxitoxin, the parent compound in the family of toxins responsible for paralytic shellfish poisoning (PSP), from a strain of the dinoflagellate Alexandrium excavatum. Quantitative results obtained by flow-injection analysis are compared to those obtained by high-performance liquid chromatography with post-column oxidation and fluorescence detection. The coupling of liquid chromatography and capillary electrophoresis with ionspray mass spectrometry is described for the separation of mixtures of PSP toxins and the highly potent pufferfish toxin tetrodotoxin. Tandem mass spectrometry is used to provide the structural information, and the ability to distinguish isomeric PSP toxins both chromatographically and mass spectrometrically is demonstrated.
离子喷雾质谱法已被用于监测从一株海洋原甲藻中提纯石房蛤毒素（导致麻痹性贝类中毒（PSP）的毒素家族中的母体化合物）的过程。将流动注射分析获得的定量结果与通过柱后氧化和荧光检测的高效液相色谱法获得的结果进行比较。描述了液相色谱和毛细管电泳与离子喷雾质谱联用用于分离PSP毒素混合物和剧毒河豚毒素的方法。串联质谱法用于提供结构信息，并展示了在色谱和质谱方面区分PSP毒素异构体的能力。
