Bowen Robin S, Gu Yang, Zhang Yanping, Lewis David F, Wang Yuping
Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA.
J Soc Gynecol Investig. 2005 Sep;12(6):428-32. doi: 10.1016/j.jsgi.2005.04.001.
Placental hypoxia and altered placental cytokine productions have been considered to play a significant role in the pathophysiology of preeclampsia. The objective of this study was to determine whether hypoxia could modify interleukin (IL)-6, IL-8, and IL-10 production by placental trophoblast cells (TCs) from normal and preeclamptic (PE) pregnancies.
Placentas were obtained from nine normal and nine PE pregnancies immediately after delivery. Placental TCs were isolated and cultured under normoxic (21% O(2)/air) and hypoxic (2% O(2)/5% CO(2)/92% N(2)) conditions for 48 hours. TC productions of IL-6, IL-8, and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA). Unpaired t tests or paired t test was used for the statistical analysis and data are expressed as means +/- SE (pg/mug cellular protein). A P value less than .05 was considered statistically significant.
PE-TCs produced significantly more IL-6, IL-8, and IL-10 than those of normal-TCs when they were cultured under normoxic condition, P < .05. Both normal-TCs and PE-TCs produced more IL-6 and IL-8 when they were cultured under hypoxic conditions. Hypoxia reduced IL-10 production by PE-TCs, but had no effect on IL-10 production by normal-TCs.
Hypoxia promotes both IL-6 and IL-8 but reduces IL-10 production by placental TCs from PE pregnancies.
胎盘缺氧及胎盘细胞因子产生的改变被认为在子痫前期的病理生理过程中起重要作用。本研究的目的是确定缺氧是否会改变正常妊娠和子痫前期（PE）妊娠胎盘滋养层细胞（TCs）白细胞介素（IL）-6、IL-8和IL-10的产生。
分娩后立即从9例正常妊娠和9例PE妊娠中获取胎盘。分离胎盘TCs并在常氧（21% O₂/空气）和缺氧（2% O₂/5% CO₂/92% N₂）条件下培养48小时。通过酶联免疫吸附测定（ELISA）测量TCs产生的IL-6、IL-8和IL-10。采用非配对t检验或配对t检验进行统计分析，数据以平均值±标准误（pg/μg细胞蛋白）表示。P值小于0.05被认为具有统计学意义。
在常氧条件下培养时，PE-TCs产生的IL-6、IL-8和IL-10明显多于正常-TCs，P < 0.05。正常-TCs和PE-TCs在缺氧条件下培养时均产生更多的IL-6和IL-8。缺氧降低了PE-TCs的IL-10产生，但对正常-TCs的IL-10产生没有影响。
缺氧促进PE妊娠胎盘TCs产生IL-6和IL-8，但降低其IL-10产生。
