Department of Physiology, Harbin Medical University, Harbin, China.
Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center - Shreveport, Shreveport, LA, USA.
Am J Reprod Immunol. 2018 Jan;79(1). doi: 10.1111/aji.12763. Epub 2017 Sep 20.
To determine if altered trophoblast CD200 and CD200R expressions promote inflammatory cytokine production in preeclamptic placentas.
Placental tissue CD200 and CD200R expressions were determined by immunostaining. Tissue sections from first-, second-, and third-trimester, normal term, and preeclamptic placentas were used. CD200 and CD200R expressions and cytokine production of TNFα, sTNFR1, INFγ, IL-4, IL-6, IL-8, and IL-10 were determined in trophoblasts from normal and preeclamptic placentas and in normal trophoblasts transfected with CD200 siRNA.
CD200, but not CD200R, expression was significantly reduced in trophoblasts from preeclamptic compared to normal placentas. Trophoblast from preeclamptic placentas and trophoblast transfected with CD200 siRNA produced significantly more TNFα, sTNFR1, IL-6, and IL-8, but significantly less IL-10, than trophoblasts from normal control placentas.
Downregulation of CD200 expression resulted in an imbalance of increased Th1 cytokine and decreased Th2 cytokine production in placental trophoblasts in preeclampsia.
确定是否改变滋养细胞 CD200 和 CD200R 的表达促进子痫前期胎盘炎症细胞因子的产生。
通过免疫染色法确定胎盘组织 CD200 和 CD200R 的表达。使用来自第一、二、三孕期、正常足月和子痫前期胎盘的组织切片。在正常和子痫前期胎盘滋养细胞以及转染 CD200 siRNA 的正常滋养细胞中，测定 TNFα、sTNFR1、INFγ、IL-4、IL-6、IL-8 和 IL-10 的 CD200 和 CD200R 表达和细胞因子产生。
与正常胎盘相比，子痫前期胎盘滋养细胞中 CD200，但不是 CD200R 的表达明显降低。与正常对照胎盘的滋养细胞相比，来自子痫前期胎盘的滋养细胞和转染 CD200 siRNA 的滋养细胞产生的 TNFα、sTNFR1、IL-6 和 IL-8 明显增加，但 IL-10 明显减少。
CD200 表达下调导致子痫前期胎盘滋养细胞中 Th1 细胞因子产生增加和 Th2 细胞因子产生减少失衡。
