一种具有类Rev特性的新型小鼠乳腺肿瘤病毒编码蛋白。

A novel, mouse mammary tumor virus encoded protein with Rev-like properties.

作者信息

Indik Stanislav, Günzburg Walter H, Salmons Brian, Rouault Francoise

机构信息

Research Institute for Virology and Biomedicine, University of Veterinary Medicine, Austria.

出版信息

Virology. 2005 Jun 20;337(1):1-6. doi: 10.1016/j.virol.2005.03.040.

DOI:10.1016/j.virol.2005.03.040

PMID:15914215

Abstract

We have identified a novel, multiple spliced, subgenomic mRNA species in MMTV producing cells of different origin containing an open reading frame encoding a 39-kDa Rev-like protein, Rem (regulator of expression of MMTV). An EGFP-Rem fusion protein is shown to be predominantly in the nucleolus. Further leptomycin B inhibits the nuclear export of nonspliced MMTV transcripts, implicating Rem in nuclear export by the Crm1 pathway in MMTV. Rem is thus reminiscent of the Rec protein from the related endogenous human retrovirus, HERV-K.

摘要

我们在不同来源的产MMTV细胞中鉴定出一种新型的、经过多次剪接的亚基因组mRNA物种，其包含一个开放阅读框，编码一种39 kDa的Rev样蛋白Rem（MMTV表达调节因子）。一种EGFP-Rem融合蛋白主要定位于核仁。此外，雷帕霉素B抑制未剪接的MMTV转录本的核输出，这表明Rem参与MMTV中通过Crm1途径的核输出。因此，Rem让人联想到来自相关内源性人类逆转录病毒HERV-K的Rec蛋白。