• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

新型小鼠乳腺肿瘤病毒（MMTV）5'顺式作用元件的人逆转录病毒转录激活子 Tat 和 Tax 的转录激活。

Transactivation of the novel 5' cis-acting element of mouse mammary tumor virus (MMTV) by human retroviral transactivators Tat and Tax.

机构信息

Department of Biochemistry and Molecular Biology, College of Medicine & Health Sciences (CMHS), United Arab Emirates (UAE) University, Al Ain, UAE.

ASPIRE Research Institute in Precision Medicine, Abu Dhabi, UAE.

出版信息

Commun Biol. 2024 Nov 16;7(1):1521. doi: 10.1038/s42003-024-07139-9.

DOI:10.1038/s42003-024-07139-9
PMID:39550519
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11569226/
Abstract

The mouse mammary tumor virus (MMTV) encodes a 5' element crucial for transcription of its genome along with the Rem/Rem-responsive element (RmRE) responsible for nuclear export of this unspliced RNA. Whether the 5' element is Rem-responsive or has any functional interaction with host/viral factors to facilitate MMTV gene expression was tested in this study. Our results reveal that the 5' element is non-responsive to Rem, but can be transactivated by both HIV Tat and HTLV-1 Tax activators. Reciprocally, MMTV could transactivate not only HIV TAR (similar to HTLV Tax), but also its 5' element. Furthermore, we reveal involvement of pTEFb, a general elongation factor associated with transactivation by Tat/Tax. This makes MMTV the first betaretrovirus to encode both Rem/RRE and Tat/TAR-Tax/TRE-like transcription regulatory systems. This study should enhance not only our understanding of retrovirus replication and virally-induced cancers/immunodeficiency syndromes, but also development of improved retroviral vectors for human gene therapy.

摘要

鼠乳腺肿瘤病毒（MMTV）编码 5' 元件，该元件对于其基因组的转录至关重要，同时还编码 Rem/Rem 反应元件（RmRE），负责该未剪接 RNA 的核输出。本研究旨在测试 5' 元件是否对 Rem 有反应，或者与宿主/病毒因子是否有任何功能相互作用，以促进 MMTV 基因表达。我们的结果表明，5' 元件对 Rem 无反应，但可被 HIV Tat 和 HTLV-1 Tax 激活剂反式激活。反过来，MMTV 不仅可以反式激活 HIV TAR（类似于 HTLV Tax），还可以反式激活其 5' 元件。此外，我们揭示了 pTEFb 的参与，pTEFb 是一种与 Tat/Tax 反式激活相关的通用延伸因子。这使得 MMTV 成为第一个编码 Rem/RRE 和 Tat/Tax/TRE 样转录调控系统的β逆转录病毒。本研究不仅应增强我们对逆转录病毒复制和病毒诱导的癌症/免疫缺陷综合征的理解，而且还应促进用于人类基因治疗的改良逆转录病毒载体的发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/6e5331a80e1b/42003_2024_7139_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/9def9e719262/42003_2024_7139_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/79fc2b10443d/42003_2024_7139_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/07a3d82634cd/42003_2024_7139_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/04eeef1856ad/42003_2024_7139_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/b3400cf5f6c6/42003_2024_7139_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/71bc6d6c818b/42003_2024_7139_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/dbc4c2ad06e3/42003_2024_7139_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/6e5331a80e1b/42003_2024_7139_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/9def9e719262/42003_2024_7139_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/79fc2b10443d/42003_2024_7139_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/07a3d82634cd/42003_2024_7139_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/04eeef1856ad/42003_2024_7139_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/b3400cf5f6c6/42003_2024_7139_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/71bc6d6c818b/42003_2024_7139_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/dbc4c2ad06e3/42003_2024_7139_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f31/11569226/6e5331a80e1b/42003_2024_7139_Fig8_HTML.jpg

相似文献

1
Transactivation of the novel 5' cis-acting element of mouse mammary tumor virus (MMTV) by human retroviral transactivators Tat and Tax.新型小鼠乳腺肿瘤病毒（MMTV）5'顺式作用元件的人逆转录病毒转录激活子 Tat 和 Tax 的转录激活。
Commun Biol. 2024 Nov 16;7(1):1521. doi: 10.1038/s42003-024-07139-9.
2
Rev and Rex proteins of human complex retroviruses function with the MMTV Rem-responsive element.人类复杂逆转录病毒的Rev和Rex蛋白与MMTV Rem反应元件共同发挥作用。
Retrovirology. 2009 Feb 3;6:10. doi: 10.1186/1742-4690-6-10.
3
A cis-Acting Element Downstream of the Mouse Mammary Tumor Virus Major Splice Donor Critical for RNA Elongation and Stability.顺式作用元件位于鼠乳腺瘤病毒主要剪接供体位点下游，对 RNA 延伸和稳定性至关重要。
J Mol Biol. 2018 Oct 19;430(21):4307-4324. doi: 10.1016/j.jmb.2018.08.025. Epub 2018 Sep 1.
4
Identification of the Rem-responsive element of mouse mammary tumor virus.小鼠乳腺肿瘤病毒Rem反应元件的鉴定。
Nucleic Acids Res. 2008 Nov;36(19):6284-94. doi: 10.1093/nar/gkn608. Epub 2008 Oct 3.
5
Selective infection of human T-lymphotropic virus type 1 (HTLV-1)-infected cells by chimeric human immunodeficiency viruses containing HTLV-1 tax response elements in the long terminal repeat.通过在长末端重复序列中含有1型人类嗜T淋巴细胞病毒（HTLV-1）tax反应元件的嵌合型人类免疫缺陷病毒对HTLV-1感染细胞进行选择性感染。
J Virol. 1995 Nov;69(11):7216-25. doi: 10.1128/JVI.69.11.7216-7225.1995.
6
Human immunodeficiency virus type 1 genome activation induced by human T-cell leukemia virus type 1 Tax protein is through cooperation of NF-kappaB and Tat.1型人类嗜T淋巴细胞病毒Tax蛋白诱导的1型人类免疫缺陷病毒基因组激活是通过核因子κB和反式激活因子的协同作用实现的。
J Virol. 1998 Aug;72(8):6911-6. doi: 10.1128/JVI.72.8.6911-6916.1998.
7
Nuclear export and expression of human T-cell leukemia virus type 1 tax/rex mRNA are RxRE/Rex dependent.人 T 细胞白血病病毒 1 型 tax/rex mRNA 的核输出和表达依赖于 RxRE/Rex。
J Virol. 2012 Apr;86(8):4559-65. doi: 10.1128/JVI.06361-11. Epub 2012 Feb 8.
8
Role of a heterologous retroviral transport element in the development of genetic complementation assay for mouse mammary tumor virus (MMTV) replication.异源逆转录病毒转运元件在小鼠乳腺肿瘤病毒（MMTV）复制基因互补分析发展中的作用。
Virology. 2009 Mar 15;385(2):464-72. doi: 10.1016/j.virol.2008.12.027. Epub 2009 Jan 20.
9
Block of Tat-mediated transactivation of tumor necrosis factor beta gene expression by polymeric-TAR decoys.通过多聚TAR诱饵阻断Tat介导的肿瘤坏死因子β基因表达的反式激活。
Virology. 1996 Aug 1;222(1):252-6. doi: 10.1006/viro.1996.0416.
10
Human T-Cell Lymphotropic Virus Type 1 Transactivator Tax Exploits the XPB Subunit of TFIIH during Viral Transcription.人类 T 细胞嗜淋巴细胞病毒 1 转录激活蛋白 Tax 在病毒转录过程中利用 TFIIH 的 XPB 亚基。
J Virol. 2020 Mar 31;94(8). doi: 10.1128/JVI.02171-19.

本文引用的文献

1
Enhanced NF-κB activation via HIV-1 Tat-TRAF6 cross-talk.通过 HIV-1 Tat-TRAF6 相互作用增强 NF-κB 激活。
Sci Adv. 2024 Jan 19;10(3):eadi4162. doi: 10.1126/sciadv.adi4162.
2
Understanding Retroviral Life Cycle and its Genomic RNA Packaging.了解逆转录病毒生命周期及其基因组RNA包装。
J Mol Biol. 2023 Feb 1;435(3):167924. doi: 10.1016/j.jmb.2022.167924. Epub 2022 Dec 16.
3
Role of RNA Polymerase II Promoter-Proximal Pausing in Viral Transcription.RNA 聚合酶 II 启动子近端暂停在病毒转录中的作用。
Viruses. 2022 Sep 13;14(9):2029. doi: 10.3390/v14092029.
4
Regulation of HTLV-1 transformation.调控 HTLV-1 转化。
Biosci Rep. 2022 Mar 31;42(3). doi: 10.1042/BSR20211921.
5
Ancient Adversary - HERV-K (HML-2) in Cancer.古老的对手——癌症中的内源性逆转录病毒K（HML-2）
Front Oncol. 2021 May 13;11:658489. doi: 10.3389/fonc.2021.658489. eCollection 2021.
6
A purine loop and the primer binding site are critical for the selective encapsidation of mouse mammary tumor virus genomic RNA by Pr77Gag.嘌呤环和引物结合位点对于 Pr77Gag 选择性包裹小鼠乳腺肿瘤病毒基因组 RNA 至关重要。
Nucleic Acids Res. 2021 May 7;49(8):4668-4688. doi: 10.1093/nar/gkab223.
7
From Entry to Egress: Strategic Exploitation of the Cellular Processes by HIV-1.从进入到逸出：HIV-1对细胞过程的策略性利用
Front Microbiol. 2020 Dec 4;11:559792. doi: 10.3389/fmicb.2020.559792. eCollection 2020.
8
The XPB Subunit of the TFIIH Complex Plays a Critical Role in HIV-1 Transcription and XPB Inhibition by Spironolactone Prevents HIV-1 Reactivation from Latency.TFIIH复合物的XPB亚基在HIV-1转录中起关键作用，螺内酯对XPB的抑制可防止HIV-1从潜伏状态重新激活。
J Virol. 2021 Feb 15;95(4). doi: 10.1128/JVI.01247-20. Epub 2020 Nov 25.
9
Human Virus Transcriptional Regulators.人类病毒转录调控因子
Cell. 2020 Jul 9;182(1):24-37. doi: 10.1016/j.cell.2020.06.023.
10
Human T-Cell Lymphotropic Virus Type 1 Transactivator Tax Exploits the XPB Subunit of TFIIH during Viral Transcription.人类 T 细胞嗜淋巴细胞病毒 1 转录激活蛋白 Tax 在病毒转录过程中利用 TFIIH 的 XPB 亚基。
J Virol. 2020 Mar 31;94(8). doi: 10.1128/JVI.02171-19.