Wakamura Sadao, Arakaki Norio, Yamamoto Masanobu, Hiradate Syuntaro, Yasui Hiroe, Kinjo Kunio, Yasuda Tetsuya, Yamazawa Hiroyuki, Ando Tetsu
Laboratory of Insect Behavior, National Institute of Agrobiological Sciences, Tsukuba.
Biosci Biotechnol Biochem. 2005 May;69(5):957-65. doi: 10.1271/bbb.69.957.
Two distinct electroantennographycally active (EAG-active) components, A and B, and a weakly active component C were found in a solvent extract from virgin females of the Ishigaki strain of the tussock moth, Orgyia postica (Walker). Components A, B, and C were found in the extract of the females at 4.0, 0.5, and 4.0 ng/female respectively. Components A, B, and C were identified as (6Z,9Z,11S,12S)-11,12-epoxyhenicosa-6,9-diene [(11S,12S)-1: posticlure], (6Z)-henicos-6-en-11-one (2), and (6Z,9Z)-henicosa-6,9-diene (3), respectively. Component B was absent in the extract from the Okinawa strain, in which components A and C were present at 2.0 and 1.5 ng/female respectively. (11S,12S)-1 and the racemic mixture showed attractiveness for both the Okinawa and Ishigaki strains, whereas (11R,12R)-1 did not. The addition of 2 significantly reduced the trap catches with (11S,12S)-1 on the Okinawa strain which lacked 2, while there was no significant inhibitory effect on the Ishigaki strain. The addition of 3 to (11S,12S)-1 did not significantly affect trap catches at Ishigaki or Okinawa. This confirmed that the attractant pheromone of O. postica of the Ishigaki strain is also (11S,12S)-1.
在冲绳草蛾石垣岛菌株未交配雌虫的溶剂提取物中，发现了两种不同的具有触角电位活性（EAG活性）的成分A和B，以及一种活性较弱的成分C。成分A、B和C在雌虫提取物中的含量分别为4.0、0.5和4.0纳克/雌虫。成分A、B和C分别被鉴定为(6Z,9Z,11S,12S)-11,12-环氧二十一碳-6,9-二烯[（11S,12S）-1：posticlure]、(6Z)-二十一碳-6-烯-11-酮（2）和(6Z,9Z)-二十一碳-6,9-二烯（3）。冲绳菌株的提取物中不存在成分B，其中成分A和C的含量分别为2.0和1.5纳克/雌虫。（11S,12S）-1和外消旋混合物对冲绳和石垣岛菌株均具有吸引力，而（11R,12R）-1则没有。添加2显著降低了缺乏2的冲绳菌株上用（11S,12S）-1诱捕到的捕获量，而对石垣岛菌株没有显著的抑制作用。在（11S,12S）-1中添加3对石垣岛或冲绳的诱捕捕获量没有显著影响。这证实了石垣岛菌株的冲绳草蛾的引诱性信息素也是（11S,12S）-1。
