Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, China.
Graduate School of Chinese Academy of Agricultural Sciences, Beijing, China.
Pest Manag Sci. 2022 Jun;78(6):2607-2617. doi: 10.1002/ps.6892. Epub 2022 Apr 20.
The tea black tussock moth Dasychira baibarana Matsumura is a devastating pest in tea plantations that causes substantial economic losses. Presently, there is no effective method to control this pest other than pesticide application. The identified sex pheromone of D. baibarana could be used for detecting and monitoring this pest.
Gas chromatography-electroantennogram detection showed that D. baibarana male moth antennae responded strongly to three components in the female sex pheromone gland, which were identified as: (3Z,6Z)-cis-9,10-epoxyhenicosa-3,6-diene (Z3,Z6,epo9-21:H) (I), (3Z,6Z,11E)-cis-9,10-epoxyhenicosa-3,6,11-triene (Z3,Z6,epo9,E11-21:H) (II) and (3Z,6Z)-henicosa-3,6-dien-11-one (Z3,Z6-21:11-one) (III). Dasychira baibarana uses a unique composition of an epoxydiene, epoxytriene, and dienone with the same 3,6-dienyl motif as its sex pheromone. The epoxytriene and dienone were not previously characterized as insect pheromone components. Electroantennogram analysis showed that each synthetic compound strongly stimulated male antennae, and compounds II and III elicited stronger responses than compound I. A wind tunnel bioassay and field trapping experiments proved that, ternary blends of compounds I-III attracted D. baibarana. Efficient attraction was achieved with a rubber septum baited with 500 μg of a mixture of compounds I-III at the ratio 25:20:55.
The three identified compounds elicited an electroantennogram response in D. baibarana male moth antennae, and a mixture of the three components at the ratio 25:20:55 attracted D. baibarana male moths in a wind tunnel assay and field trapping experiments. This blend could be used for integrated management of D. baibarana in tea plantations. © 2022 Society of Chemical Industry.
茶黑毒蛾 Dasychira baibarana Matsumura 是茶园中一种极具破坏性的害虫，会造成巨大的经济损失。目前，除了使用农药外，尚无其他有效方法来控制这种害虫。已经鉴定出的 D. baibarana 性信息素可用于检测和监测这种害虫。
气相色谱-触角电位检测显示，D. baibarana 雄蛾触角对雌蛾性信息素腺体中的三种成分反应强烈，这三种成分分别鉴定为：（3Z,6Z）-顺-9,10-环氧二十碳-3,6-二烯（Z3,Z6,epo9-21:H）（I）、（3Z,6Z,11E）-顺-9,10-环氧二十碳-3,6,11-三烯（Z3,Z6,epo9,E11-21:H）（II）和（3Z,6Z）-二十碳-3,6-二烯-11-酮（Z3,Z6-21:11-one）（III）。D. baibarana 利用一种独特的环氧二烯、环氧三烯和二烯酮组成的混合物作为性信息素，其 3,6-二烯基结构相同。以前没有将环氧三烯和二烯酮鉴定为昆虫信息素成分。触角电位分析表明，每种合成化合物都强烈刺激了雄蛾的触角，化合物 II 和 III 比化合物 I 产生了更强的反应。风洞生物测定和田间诱捕实验证明，化合物 I-III 的三元混合物吸引了 D. baibarana。在风洞试验和田间诱捕试验中，用 500μg 的混合物（化合物 I-III 的比例为 25:20:55）涂在橡胶隔垫上，能有效地吸引 D. baibarana。
在 D. baibarana 雄蛾触角上，三种鉴定出的化合物都能引起触角电位反应，而在风洞试验和田间诱捕试验中，三种成分的混合物（比例为 25:20:55）能吸引 D. baibarana 雄蛾。这种混合物可用于茶园中 D. baibarana 的综合治理。 © 2022 英国化学学会。
