Takenaka Shinji, Setyorini Endang, Kim Young-Ju, Murakami Shuichiro, Aoki Kenji
Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Kobe, Japan.
Biosci Biotechnol Biochem. 2005 May;69(5):1033-5. doi: 10.1271/bbb.69.1033.
Pseudomonas sp. strain AP-3 grows on benzoate, p-hydroxybenzoate, protocatechuate, and 2-aminophenol as sole carbon and energy source. This strain converted benzoate and p-hydroxybenzoate to catechol and protocatechuate respectively, which were metabolized via the ortho-cleavage pathway. The enzymes responsible for these reactions were shown to be inducible. In contrast, strain AP-3 constitutively expresses the enzymes involved in the metabolism of 2-aminophenol.
假单胞菌属菌株AP-3能以苯甲酸盐、对羟基苯甲酸盐、原儿茶酸和2-氨基苯酚作为唯一碳源和能源生长。该菌株分别将苯甲酸盐和对羟基苯甲酸盐转化为儿茶酚和原儿茶酸，它们通过邻位裂解途径进行代谢。负责这些反应的酶被证明是可诱导的。相比之下，菌株AP-3组成型表达参与2-氨基苯酚代谢的酶。
