Pyrhönen S, Tiainen M, Rautonen J, Tammilehto L, Laasonen A, Mattson K, Knuutila S
Department of Radiotherapy and Oncology, Helsinki University Central Hospital, Finland.
Cancer Genet Cytogenet. 1992 May;60(1):8-13. doi: 10.1016/0165-4608(92)90224-v.
The correlation between flow cytometric analysis and classical karyotyping was examined in malignant mesothelioma. Flow cytometry was used to determine the DNA ploidy mainly from paraffin blocks of 31 mesotheliomas, of which the S-phase fraction (SPF) could be defined in 17 tumors. Chromosomal results were available from 27 of these tumors. For the comparisons, both the modal (the most common) and the mean (the average) chromosome numbers were determined. A significant correlation was observed between ploidy pattern, i.e., DNA indexes and modal as well as mean chromosome numbers (p = 0.004 and p = 0.006, respectively). Otherwise the comparison between the mean chromosome numbers and DNA index proved more relevant. Although the majority of the tumors (16 of 27) had a normal modal chromosome number of 46, only 5 tumors had a normal mean chromosome number, and the remaining 22 had an abnormal number, reflecting the divergent chromosomal abnormalities. Furthermore, comparison of SPF with mean chromosome numbers disclosed a parabolic relationship; i.e., when mean chromosome number corresponded to diploid, near-diploid, or near-tetraploid cell, SPF was low, but SPF was at the maximum when mean chromosome number ranged between 60 and 63. Because SPF is an indicator of cell proliferation, this might suggest that the number of chromosomes as such also corresponds with the growth characteristics of malignant mesothelioma.
在恶性间皮瘤中检测了流式细胞术分析与经典核型分析之间的相关性。流式细胞术主要用于从31例间皮瘤的石蜡块中确定DNA倍性，其中17例肿瘤可定义S期分数（SPF）。这些肿瘤中有27例可获得染色体结果。为了进行比较，确定了众数（最常见的）和平均（平均）染色体数。在倍性模式，即DNA指数与众数以及平均染色体数之间观察到显著相关性（分别为p = 0.004和p = 0.006）。否则，平均染色体数与DNA指数之间的比较被证明更具相关性。尽管大多数肿瘤（27例中的16例）的众数染色体数正常，为46，但只有5例肿瘤的平均染色体数正常，其余22例染色体数异常，反映了不同的染色体异常情况。此外，SPF与平均染色体数的比较揭示了一种抛物线关系；即当平均染色体数对应于二倍体、近二倍体或近四倍体细胞时，SPF较低，但当平均染色体数在60至63之间时，SPF最高。由于SPF是细胞增殖的指标，这可能表明染色体数量本身也与恶性间皮瘤的生长特征相对应。
