Seidlová-Wuttke D, Jarry H, Pitzel L, Wuttke W
Department of Clinical and Experimental Endocrinology, University of Göttingen, Göttingen, Germany.
Maturitas. 2005 Jun 16;51(2):177-86. doi: 10.1016/j.maturitas.2004.07.007.
Estradiol (E2) and testosterone (T) effectively prevent orchidectomy (orx) induced osteoporosis. T, however, stimulates prostate proliferation which may lead to malignancy. We showed that a Cimicifuga racemosa (CR) preparation had bone-sparing effects without exerting estrogenic effects in the uterus. We studied therefore whether a CR preparation has also antiosteoporotic effects in orx rats substituted with E2, T or CR via pelleted food over a period of 3 months. Average daily intake per animal was: T: 25 mg; E2: 0.325 mg, CR low dose: 33 mg; CR high dose: 133 mg. E2, T and CR at the high dose partially prevented development of osteoporosis as measured by quantitative computer tomography in the metaphysis of the tibia. E2, but not T or CR reduced serum osteocalcin and the metabolic products of collagen-1alpha1. Gene expression of collagen-1alpha1 and tartrate-resistant acid phosphatase was decreased by E2 and the higher dose of the CR extract but increased in the T-treated animals. In the prostate T inhibited androgen receptor, estrogen receptor alpha and insulin-like growth factor-1 gene expression but stimulated the expression of the ERbeta gene. These effects were not shared by E2 or both doses of the CR extract. It is concluded that E2, T and CR exert antiosteoporotic effects in the metaphysis of the tibia of orx rats. T has profound effects in the prostate which were not seen in the E2- and CR-treated animals. Therefore, the Cimicifuga racemosa extract BNO 1055 may be useful to prevent osteoporosis in aged male patients with reduced testosterone production.
雌二醇（E2）和睾酮（T）可有效预防去势（orx）诱导的骨质疏松症。然而，T会刺激前列腺增生，这可能导致恶性肿瘤。我们发现，升麻（CR）制剂具有保护骨骼的作用，且对子宫无雌激素样作用。因此，我们研究了一种CR制剂对通过颗粒饲料给予E2、T或CR替代的去势大鼠在3个月内是否也具有抗骨质疏松作用。每只动物的平均每日摄入量为：T：25毫克；E2：0.325毫克，CR低剂量：33毫克；CR高剂量：133毫克。通过定量计算机断层扫描测量胫骨干骺端，高剂量的E2、T和CR可部分预防骨质疏松症的发展。E2可降低血清骨钙素和胶原蛋白-1α1的代谢产物，但T或CR则无此作用。E2和较高剂量的CR提取物可降低胶原蛋白-1α1和抗酒石酸酸性磷酸酶的基因表达，但T处理的动物中这些基因表达增加。在前列腺中，T抑制雄激素受体、雌激素受体α和胰岛素样生长因子-1基因的表达，但刺激ERβ基因的表达。E2或两种剂量的CR提取物均未出现这些作用。得出的结论是，E2、T和CR对去势大鼠胫骨干骺端具有抗骨质疏松作用。T对前列腺有深远影响，而在E2和CR处理的动物中未观察到这种影响。因此，升麻提取物BNO 1055可能有助于预防睾酮分泌减少的老年男性患者的骨质疏松症。
