Qin Mingde, Tai Guangping, Collas Philippe, Polak Julia M, Bishop Anne E
Tissue Engineering & Regenerative Medicine Centre, Imperial College Faculty of Medicine, Chelsea & Westminster Campus, Fulham Road, London SW10 9NH, UK.
Stem Cells. 2005 Jun-Jul;23(6):712-8. doi: 10.1634/stemcells.2004-0195.
Various means have been used to encourage the differentiation of embryonic stem cells (ESCs) toward specific lineages, including growth factor administration, genetic modification, and coculture with relevant cells/tissues. Cell extract-based reprogramming has recently been used to derive mature cells from nonrelated phenotypes. In this communication, we tested whether this in vitro reprogramming approach can be used to direct ESC differentiation. Permeabilized murine ESCs exposed to extracts of murine type II pneumocytes showed increased expression of surfactant protein C and its corresponding mRNA, reflecting enhanced differentiation of pneumocytes. Subsequent differentiation to a type I phenotype was demonstrated by expression of aquaporin 5. Pneumocyte formation occurred quicker than with growth factor-induced differentiation. Our findings establish that ESCs can be differentiated in vitro using cellular extracts. This model provides a tool for analysis of the key factors involved in the differentiation of ESCs to type II pneumocytes.
人们已经采用了各种方法来促使胚胎干细胞（ESC）向特定谱系分化，包括施用生长因子、进行基因改造以及与相关细胞/组织共培养。基于细胞提取物的重编程方法最近已被用于从非相关表型中获得成熟细胞。在本通讯中，我们测试了这种体外重编程方法是否可用于指导ESC分化。暴露于小鼠II型肺上皮细胞提取物的通透化小鼠ESC显示表面活性蛋白C及其相应mRNA的表达增加，这反映了肺上皮细胞分化增强。水通道蛋白5的表达证明随后向I型表型的分化。肺上皮细胞的形成比生长因子诱导的分化更快。我们的研究结果表明，ESC可利用细胞提取物在体外进行分化。该模型为分析参与ESC向II型肺上皮细胞分化的关键因素提供了一种工具。
