Wen Zhiyou, Liao Wei, Chen Shulin
Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USA.
Appl Biochem Biotechnol. 2005 Spring;121-124:93-104.
A cellulase production process was developed by growing the fungi Trichoderma reesei and Aspergillus phoenicis on dairy manure. T. reesei produced a high total cellulase titer (1.7 filter paper units [FPU]/mL, filter paper activity) in medium containing 10 g/L of manure (dry basis [w/w]), 2 g/L KH2PO4, 2 mL/L of Tween-80, and 2mg/L of CoCl2. However, beta-glucosidase activity in the T. reesei-enzyme system was very low. T. reesei was then cocultured with A. phoenicis to enhance the beta-glucosidase level. The mixed culture resulted in a relatively high level of total cellulase (1.54 FPU/mL) and beta-glucosidase (0.64 IU/mL). The ratio of beta-glucosidase activity to filter paper activity was 0.41, suitable for hydrolyzing manure cellulose. The crude enzyme broth from the mixed culture was used for hydrolyzing the manure cellulose, and the produced glucose was significantly (p < 0.01) higher than levels obtained by using the commercial enzyme or the enzyme broth of the pure culture T. reesei.
通过在奶牛粪便上培养里氏木霉和嗜热栖热放线菌开发了一种纤维素酶生产工艺。里氏木霉在含有10 g/L粪便（干基[w/w]）、2 g/L KH2PO4、2 mL/L吐温80和2mg/L CoCl2的培养基中产生了较高的总纤维素酶滴度（1.7滤纸单位[FPU]/mL，滤纸活性）。然而，里氏木霉酶系统中的β-葡萄糖苷酶活性非常低。然后将里氏木霉与嗜热栖热放线菌共培养以提高β-葡萄糖苷酶水平。混合培养产生了相对较高水平的总纤维素酶（1.54 FPU/mL）和β-葡萄糖苷酶（0.64 IU/mL）。β-葡萄糖苷酶活性与滤纸活性的比率为0.41，适合水解粪便纤维素。混合培养的粗酶液用于水解粪便纤维素，产生的葡萄糖显著（p < 0.01）高于使用商业酶或里氏木霉纯培养酶液获得的水平。
