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用真菌灰腐质霉的重组木聚糖酶和商业Cartazyme HS木聚糖酶对有机溶剂法甘蔗渣浆进行酶法漂白。

Enzymatic bleaching of organosolv sugarcane bagasse pulps with recombinant xylanase of the fungus Humicola grisea and with commercial Cartazyme HS xylanase.

作者信息

Moriya Regina Y, Gonçalves Adilson R, Faria Fabrícia P

机构信息

Departamento de Biotecnologia, FAENQUIL, CP 116, CEP 12600-970, Lorena, SP, Brazil.

出版信息

Appl Biochem Biotechnol. 2005 Spring;121-124:195-203. doi: 10.1385/abab:121:1-3:0195.

DOI:10.1385/abab:121:1-3:0195
PMID:15917599
Abstract

Organosolv (ethanol/water and acetosolv) pulps were treated with Humicola grisea var. thermoidea and compared with Cartazyme HS xylanase-treated pulp. The ethanol/water pulps treated with H. grisea had the same viscosity as unbleached pulps (8 cP). Ethanol/water pulps treated with Cartazyme had higher viscosity than H. grisea-treated pulps (12 cP). Acetosolv pulps treated with H. grisea and Cartazyme presented a reduction in viscosity; however, the pulps treated with H. grisea had a lower reduction in viscosity than Cartazyme-treated pulps. Ethanol/water pulps treated with H. grisea had a 23% reduction in kappa number in 4 and 8 h of treatment, compared with the unbleached pulps. Cartazyme-treated pulps had a kappa number similar to that of the control pulps for 4 h of treatment. Extending the treatment time to 12 h resulted in a reduction of 33%. The acetosolv pulp treated with H. grisea had a kappa number reduced to 23% in 4 h. Cartazyme treatment resulted in a reduction of 55 and 44% in kappa number for 4 and 8 h of treatment, respectively, when compared with control pulp. Extending the treatment time to 12 h decreased the kappa number 72%. Fourier transform infrared spectra and principal component analysis showed differences among unbleached, H. grisea-treated, and Cartazyme-treated pulps.

摘要

用嗜热栖热放线菌处理有机溶剂制浆(乙醇/水和乙酸制浆),并与用Cartazyme HS木聚糖酶处理的纸浆进行比较。用嗜热栖热放线菌处理的乙醇/水纸浆与未漂白纸浆具有相同的粘度(8厘泊)。用Cartazyme处理的乙醇/水纸浆比用嗜热栖热放线菌处理的纸浆具有更高的粘度(12厘泊)。用嗜热栖热放线菌和Cartazyme处理的乙酸制浆粘度均有所降低;然而,用嗜热栖热放线菌处理的纸浆粘度降低幅度低于用Cartazyme处理的纸浆。与未漂白纸浆相比,用嗜热栖热放线菌处理的乙醇/水纸浆在处理4小时和8小时后卡伯值降低了23%。用Cartazyme处理的纸浆在处理4小时时卡伯值与对照纸浆相似。将处理时间延长至12小时,卡伯值降低了33%。用嗜热栖热放线菌处理的乙酸制浆在4小时内卡伯值降至23%。与对照纸浆相比,用Cartazyme处理的纸浆在处理4小时和8小时后卡伯值分别降低了55%和44%。将处理时间延长至12小时,卡伯值降低了72%。傅里叶变换红外光谱和主成分分析表明,未漂白纸浆、用嗜热栖热放线菌处理的纸浆和用Cartazyme处理的纸浆之间存在差异。

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