Moriya Regina Y, Gonçalves Adilson R, Duarte Marta C T
Departamento de Biotecnologia, FAENQUIL, PO Box 116, Lorena 12600-970, SP, Brazil.
Appl Biochem Biotechnol. 2005 Spring;121-124:171-81. doi: 10.1385/abab:121:1-3:0171.
Organosolv (ethanol/water and acetosolv) pulps were treated with Bacillus pumilus xylanase for 4, 8, and 12 h and compared with commercial Cartazyme HS xylanase-treated pulps. Treatment of ethanol/water pulps with B. pumilus xylanase increased viscosity by 40% in 8 h of treatment compared with pulps treated without enzyme. However, acetosolv pulps treated with B. pumilus xylanase lost viscosity. Ethanol/water pulps treated with Cartazyme had a viscosity of 18.5 cP in 4 h of treatment. In the acetosolv pulps treated with commercial enzyme, the loss of viscosity was 20% compared with pulps treated without enzyme. Ethanol/water pulps treated with B. pumilus and Cartazyme had similar effects: a 44% reduction in kappa number for pulps treated with enzyme followed by alkaline extraction compared with pulps treated with alkaline extraction. In acetosolv pulps treated with B. pumilus, the kappa number was from 12 to 18, compared with pulps treated without enzyme, which had a 40% reduction in 4 and 12 h and a 60% reduction in 8 h. Cartazyme-treated acetosolv pulps had a kappa number of 14 in 4 and 8 h of treatment. For 12 h of treatment, the kappa number was 8. Fourier transform infrared spectra of the pulps showed that enzyme-treated pulps had changes in the 1000 cm-1 absorption owing to a C-O bond present in esters. Using principal component analysis, it is possible to differentiate the unbleached pulps and enzyme-treated pulps.
用短小芽孢杆菌木聚糖酶对有机溶剂制浆（乙醇/水和乙酸制浆）处理4小时、8小时和12小时，并与经商业Cartazyme HS木聚糖酶处理的纸浆进行比较。用短小芽孢杆菌木聚糖酶处理乙醇/水纸浆，与未用酶处理的纸浆相比，在处理8小时后粘度增加了40%。然而，用短小芽孢杆菌木聚糖酶处理的乙酸制浆纸浆粘度下降。用Cartazyme处理的乙醇/水纸浆在处理4小时后粘度为18.5厘泊。在用商业酶处理的乙酸制浆纸浆中，与未用酶处理的纸浆相比，粘度损失为20%。用短小芽孢杆菌和Cartazyme处理的乙醇/水纸浆有相似的效果：与仅进行碱萃取处理的纸浆相比，经酶处理后再进行碱萃取的纸浆卡伯值降低了44%。在用短小芽孢杆菌处理的乙酸制浆纸浆中，卡伯值在12至18之间，而未用酶处理的纸浆在4小时和12小时时卡伯值降低了40%，在8小时时降低了60%。经Cartazyme处理的乙酸制浆纸浆在处理4小时和8小时时卡伯值为14。处理12小时后，卡伯值为8。纸浆的傅里叶变换红外光谱表明，经酶处理的纸浆由于酯中存在的C-O键，在1000 cm-1处的吸收发生了变化。使用主成分分析，可以区分未漂白纸浆和经酶处理的纸浆。
