Younger J Michael, Fan Chun-Yang, Chen Liling, Rosser Meredith F N, Patterson Cam, Cyr Douglas M
Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, USA.
Methods Mol Biol. 2005;301:293-303. doi: 10.1385/1-59259-895-1:293.
Components of the ubiquitin-proteasome system function on the surface of the endoplasmic reticulum (ER) to select misfolded proteins for degradation. Herein we describe methods that allow for the study of the pathway for proteasomal degradation of the cystic fibrosis transmembrane conductance regulator (CFTR). The experimental system described employs transiently transfected HEK-293 cells and is utilized to monitor the biogenesis of CFTR by Western blot and pulse-chase analysis.
泛素-蛋白酶体系统的组分在内质网（ER）表面发挥作用，以选择错误折叠的蛋白质进行降解。在此，我们描述了一些方法，这些方法可用于研究囊性纤维化跨膜传导调节因子（CFTR）的蛋白酶体降解途径。所描述的实验系统采用瞬时转染的HEK-293细胞，并用于通过蛋白质免疫印迹和脉冲追踪分析监测CFTR的生物合成。
