SUMO化蛋白的检测
Detection of sumoylated proteins.
作者信息
Hilgarth Roland S, Sarge Kevin D
机构信息
Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, USA.
出版信息
Methods Mol Biol. 2005;301:329-38. doi: 10.1385/1-59259-895-1:329.
Abstract
Small ubiquitin-related modifier (SUMO) is an ubiquitin-like protein that is covalently attached to a variety of target proteins. Unlike ubiquitination, sumoylation does not target proteins for proteolytic breakdown, but is involved in regulation of protein function, nuclear targeting, and the formation of subcellular structures. Because SUMO is involved in such a plethora of functions and modifies numerous proteins it is important to identify proteins that are sumoylated in order to increase our understanding of how this modification affects protein function and localization. This overview describes techniques utilized for the detection of sumoylated proteins. The techniques covered include immunoprecipitation, an in vitro sumoylation assay, and gel shift mobility assays that have been used to identify SUMO-modified proteins.
摘要
小泛素相关修饰物（SUMO）是一种类泛素蛋白，可共价连接到多种靶蛋白上。与泛素化不同，SUMO化并不将蛋白质作为蛋白水解降解的靶点，而是参与蛋白质功能的调节、核靶向以及亚细胞结构的形成。由于SUMO参与了如此众多的功能并修饰了大量蛋白质，因此识别被SUMO化的蛋白质对于增进我们对这种修饰如何影响蛋白质功能和定位的理解非常重要。本综述描述了用于检测SUMO化蛋白质的技术。所涵盖的技术包括免疫沉淀、体外SUMO化测定以及用于鉴定SUMO修饰蛋白质的凝胶迁移率测定。
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