Cools-Lartigue Jonathan, Marshall Jean Claude, Caissie Amanda L, Saraiva Vinicius S, Burnier Miguel N
3775 University Lyman Duff, Rm 216, Montreal, QC, H3A 2B4, Canada.
Melanoma Res. 2005 Jun;15(3):141-5. doi: 10.1097/00008390-200506000-00001.
Host-tumour interactions in uveal melanoma, and their involvement in the biological events leading to metastasis and eventually mortality, are not well understood. It is known that uveal melanoma disseminates predominantly via a haematogenous route with metastasis developing primarily in the liver. Therefore, cytokines involved in angiogenesis, such as vascular endothelial growth factor (VEGF), and those expressed in large quantities within the liver, such as hepatocyte growth factor (HGF), are of particular interest in uveal melanoma research. This study investigated the levels of HGF and VEGF in monocyte and uveal melanoma-conditioned medium. Five human uveal melanoma cell lines and one monocyte cell line were seeded in six-well plates. After 18 h, melanoma-conditioned medium (MCM) was placed on the monocyte cell line and monocyte-conditioned medium (MoCM) was placed on each uveal melanoma cell line. Tumour cells and monocytes incubated in fresh, as opposed to conditioned, medium after 18 h were used as controls. VEGF and HGF levels were determined by immunoassay prior to media transfer and 6, 12, 24 and 36 h thereafter. Both cytokines showed an upregulation of expression from all cells after incubation in conditioned medium. 28SC incubated in MCM expressed higher levels of the given cytokines than did uveal melanoma cells incubated in MoCM. In addition, each cell line exhibited a distinct pattern of expression, with individual cell lines exhibiting different peak levels of cytokine production at different time points. These results offer insight into the upregulation of VEGF and HGF, which may play a role in tumour-host cell interactions.
葡萄膜黑色素瘤中的宿主 - 肿瘤相互作用，以及它们在导致转移并最终导致死亡的生物学事件中的作用，目前尚未完全了解。已知葡萄膜黑色素瘤主要通过血行途径播散，转移主要发生在肝脏。因此，参与血管生成的细胞因子，如血管内皮生长因子（VEGF），以及在肝脏中大量表达的细胞因子，如肝细胞生长因子（HGF），在葡萄膜黑色素瘤研究中特别受关注。本研究调查了单核细胞和葡萄膜黑色素瘤条件培养基中HGF和VEGF的水平。将五种人葡萄膜黑色素瘤细胞系和一种单核细胞系接种于六孔板中。18小时后，将黑色素瘤条件培养基（MCM）置于单核细胞系上，将单核细胞条件培养基（MoCM）置于每种葡萄膜黑色素瘤细胞系上。18小时后在新鲜培养基（而非条件培养基）中孵育的肿瘤细胞和单核细胞用作对照。在培养基转移前以及之后6、12、24和36小时，通过免疫测定法测定VEGF和HGF水平。在条件培养基中孵育后，两种细胞因子在所有细胞中的表达均上调。在MCM中孵育的28SC表达的给定细胞因子水平高于在MoCM中孵育的葡萄膜黑色素瘤细胞。此外，每个细胞系都表现出独特的表达模式，各个细胞系在不同时间点表现出不同的细胞因子产生峰值水平。这些结果为VEGF和HGF的上调提供了见解，这可能在肿瘤 - 宿主细胞相互作用中发挥作用。
