Ocular Oncology Service, Hacettepe University School of Medicine, Ankara, Turkey.
Melanoma Res. 2010 Apr;20(2):126-32. doi: 10.1097/CMR.0b013e328335a916.
This study evaluated the expression profile of hepatocyte growth factor (HGF), c-Met, epidermal growth factor receptor (EGFR), insulin-like growth factor-1 receptor (IGF-1R), and vitreal and serum EGF, HGF, IGF-1 levels in patients with uveal melanoma and assessed their correlation with the clinicopathological parameters. Forty patients with uveal melanoma were included in the study. Clinicopathological parameters were evaluated with hematoxylin-eosin staining. HGF, c-Met, EGFR, and IGF-1R expressions were evaluated immunohistochemically. HGF, EGF, and IGF-1 levels were measured with enzyme-linked immunosorbent assay in vitreous and serum specimens taken at enucleation and 6 months after the enucleation. HGF, c-Met, IGF-1R, and EGFR expressions were detected in 57.5, 20, 20, and 12.5% of cases, respectively. IGF-1R expression was significantly correlated with the degree of pigmentation, necrosis, and lymphocyte infiltration (P=0.013, 0.04, and 0.017). EGFR expression was significantly correlated with the mitosis rate (P=0.02). Vitreal EGF and serum IGF-1 levels were significantly higher in patients with scleral invasion (15.72+/-29.13, 199.01+/-154.01 pg/ml, respectively) when compared with the patients without invasion (0.56+/-1.05, 33.01+/-36.52 pg/ml) (P=0.03 and 0.015). When the preoperative and postoperative serum growth factor levels were compared, the serum EGF level was found to be lower (125.93+/-62.84, 100.02+/-31.19 pg/ml, P=0.007) and the serum IGF-1 level (165.81+/-153.6, 301.35+/-131.24 pg/ml, P<0.001) was found to be higher in the postoperative 6-month specimens. Uveal melanomas express HGF, c-Met, EGFR, and IGF-1R. Vitreal growth factor levels and expression of EGFR and IGF-1R are correlated with some clinicopathological parameters. IGF-1 and EGF may have a role in the development and progression of uveal melanoma.
本研究评估了肝细胞生长因子（HGF）、c-Met、表皮生长因子受体（EGFR）、胰岛素样生长因子-1 受体（IGF-1R）在葡萄膜黑色素瘤患者中的表达谱，并评估了它们与临床病理参数的相关性。将 40 例葡萄膜黑色素瘤患者纳入研究。通过苏木精-伊红染色评估临床病理参数。通过免疫组织化学评估 HGF、c-Met、EGFR 和 IGF-1R 的表达。通过酶联免疫吸附试验在眼球摘出术时和眼球摘出术后 6 个月采集的玻璃体和血清标本中测量 HGF、EGF、IGF-1 水平。在 57.5%、20%、20%和 12.5%的病例中检测到 HGF、c-Met、IGF-1R 和 EGFR 的表达。IGF-1R 的表达与色素沉着、坏死和淋巴细胞浸润程度显著相关（P=0.013、0.04 和 0.017）。EGFR 的表达与有丝分裂率显著相关（P=0.02）。与无侵犯患者（0.56+/-1.05、33.01+/-36.52 pg/ml）相比，侵犯巩膜的患者（15.72+/-29.13、199.01+/-154.01 pg/ml）的玻璃体 EGF 和血清 IGF-1 水平显著升高（P=0.03 和 0.015）。当比较术前和术后血清生长因子水平时，发现血清 EGF 水平降低（125.93+/-62.84、100.02+/-31.19 pg/ml，P=0.007），血清 IGF-1 水平升高（165.81+/-153.6、301.35+/-131.24 pg/ml，P<0.001）。葡萄膜黑色素瘤表达 HGF、c-Met、EGFR 和 IGF-1R。玻璃体生长因子水平以及 EGFR 和 IGF-1R 的表达与一些临床病理参数相关。IGF-1 和 EGF 可能在葡萄膜黑色素瘤的发生和发展中起作用。
