Quatresooz Pascale, Piérard Gérald E
Department of Dermatopathology, University Hospital Sart Tilman, B-4000 Liège, Belgium.
Melanoma Res. 2005 Jun;15(3):161-8. doi: 10.1097/00008390-200506000-00004.
Cells of melanocytic naevi and cutaneous malignant melanomas (MM) are surrounded by a basement membrane (BM).
To scrutinize any difference between the deposits of alpha1 (IV) and alpha5 (IV) collagen chains in melanocytic naevi and MM.
A total of 27 common melanocytic naevi, 11 dysplastic naevi, 21 atypical naevi (melanocytomas) including Spitz and non-Spitz types, as well as 24 MM were studied. Their phenotypic and functional characteristics defined by immunohistochemistry using a panel of antibodies, including those directed to the alpha1 (IV), alpha3 (IV) and alpha5 (IV) collagen chains.
Almost all naevi and half the melanocytomas exhibited a strong positivity for the alpha1 (IV) collagen chain. By contrast, the remaining melanocytomas and MM presented a heterogeneous staining pattern for the alpha1 (IV) collagen chain. One third of the naevi, 23% of the MM without cutaneous micrometastasis and 83% of MM with cutaneous micrometastasis showed discrete cytoplasmic positivity for the alpha5 (IV) collagen chain. All other melanocytic tumours were negative for this antibody. Rare MM cells in transepidermal migration were stained with the anti-alpha1 (IV) or alpha5 (IV) collagen chain antibodies. No immunoreactivity for the alpha3 (IV) collagen chain was disclosed in any of the samples.
We report the expression of alpha1 (IV) and alpha5 (IV) collagen chains in naevi and MM. The inconsistent staining pattern for alpha1 (IV) collagen chain in phenotypically atypical melanocytomas and in MM highlight the heterogeneity in both cell differentiation and stroma-tumour interactions. This biological aspect may be related to neoplastic progression and influence metastatic potential.
黑素细胞痣和皮肤恶性黑色素瘤（MM）的细胞被基底膜（BM）所包围。
仔细研究黑素细胞痣和MM中α1（IV）和α5（IV）胶原链沉积之间的差异。
共研究了27例普通黑素细胞痣、11例发育异常痣、21例非典型痣（黑素细胞瘤），包括Spitz型和非Spitz型，以及24例MM。使用一组抗体通过免疫组织化学确定它们的表型和功能特征，这些抗体包括针对α1（IV）、α3（IV）和α5（IV）胶原链的抗体。
几乎所有的痣和一半的黑素细胞瘤对α1（IV）胶原链呈强阳性。相比之下，其余的黑素细胞瘤和MM对α1（IV）胶原链呈现异质性染色模式。三分之一的痣、23%无皮肤微转移的MM和83%有皮肤微转移的MM对α5（IV）胶原链显示离散的细胞质阳性。所有其他黑素细胞肿瘤对该抗体均为阴性。经表皮迁移的罕见MM细胞用抗α1（IV）或α5（IV）胶原链抗体染色。在任何样本中均未发现α3（IV）胶原链的免疫反应性。
我们报道了痣和MM中α1（IV）和α5（IV）胶原链的表达。表型非典型黑素细胞瘤和MM中α1（IV）胶原链染色模式的不一致突出了细胞分化和基质-肿瘤相互作用中的异质性。这一生物学方面可能与肿瘤进展有关并影响转移潜能。
