鸟氨酸脱羧酶、丝裂原活化蛋白激酶和基质金属蛋白酶-2在人结肠肿瘤中的表达
Ornithine decarboxylase, mitogen-activated protein kinase and matrix metalloproteinase-2 expressions in human colon tumors.
作者信息
Nemoto Takahiro, Kubota Shunichiro, Ishida Hideyuki, Murata Nobuo, Hashimoto Daijo
机构信息
Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
出版信息
World J Gastroenterol. 2005 May 28;11(20):3065-9. doi: 10.3748/wjg.v11.i20.3065.
AIM
To investigate the expressions of ornithine decarboxylase (ODC), MMP-2, and Erk, and their relationship in human colon tumors.
METHODS
ODC activity, MMP-2 expression, and mitogen-activated protein (MAP) kinase activity (Erk phosphorylation) were determined in 58 surgically removed human colon tumors and their adjacent normal tissues, using [1-14C]-ornithine as a substrate, ELISA assay, and Western blotting, respectively.
RESULTS
ODC activity, MMP-2 expression, and Erk phosphorylation were significantly elevated in colon tumors, compared to those in adjacent normal tissues. A significant correlation was observed between ODC activities and MMP-2 levels.
CONCLUSION
This is the first report showing a significant correlation between ODC activities and MMP-2 levels in human colon tumors. As MMP-2 is involved in cancer invasion and metastasis, and colon cancer overexpresses ODC, suppression of ODC expression may be a rational approach to treat colon cancer which overexpresses ODC.
目的
研究鸟氨酸脱羧酶（ODC）、基质金属蛋白酶-2（MMP-2）和细胞外信号调节激酶（Erk）在人结肠肿瘤中的表达及其相互关系。
方法
分别以[1-14C]-鸟氨酸为底物、采用酶联免疫吸附测定法（ELISA）及蛋白质印迹法，检测58例手术切除的人结肠肿瘤及其癌旁正常组织中的ODC活性、MMP-2表达和丝裂原活化蛋白（MAP）激酶活性（Erk磷酸化）。
结果
与癌旁正常组织相比，结肠肿瘤中的ODC活性、MMP-2表达和Erk磷酸化显著升高。ODC活性与MMP-2水平之间存在显著相关性。
结论
本研究首次报道了人结肠肿瘤中ODC活性与MMP-2水平之间存在显著相关性。由于MMP-2参与癌症侵袭和转移，且结肠癌中ODC过表达，抑制ODC表达可能是治疗ODC过表达结肠癌的合理方法。
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