Pagoria D, Geurtsen W
Department of Restorative Dentistry/Division of Operative Dentistry, School of Dentistry, University of Washington, Box 357456, Seattle, WA, 98195-7456 USA.
Biomaterials. 2005 Nov;26(31):6136-42. doi: 10.1016/j.biomaterials.2005.04.001.
Recent studies have revealed that visible-light (VL)-irradiated camphorquinone (CQ), in the presence of a tertiary amine (e.g., N,N-dimethyl-p-toluidine, DMT), generates initiating radicals that may indiscriminately react with molecular oxygen forming reactive oxygen species (ROS). In this study, the ability of the antioxidants N-acetyl-l-cysteine (NAC) and ascorbic acid (AA) to reduce intracellular oxidative stress induced by VL-irradiated CQ/DMT or VL-irradiated hydrogen peroxide (H(2)O(2)) was assessed in an immortalized Murine cementoblast cell line (OCCM.30) and an immortalized Murine fibroblast cell line, 3T3-Swiss albino (3T3). Intracellular oxidative stress was measured with the membrane permeable dye, 2',7'-dichlorodihydrofluorescein diacetate (H(2)DCF-DA). VL-irradiated CQ/DMT and VL-irradiated H(2)O(2) each produced significantly (p<0.001) elevated intracellular oxidative levels in both cell types compared to intracellular ROS levels in VL-irradiated untreated cells. OCCM.30 cementoblasts were found to be almost twice as sensitive to VL-irradiated CQ/DMT and VL-irradiated H(2)O(2) treatment compared to 3T3 fibroblasts. Furthermore, 10mm NAC and 10mm AA each eliminated oxidative stress induced by VL-irradiated CQ/DMT and VL-irradiated H(2)O(2) in both cell types. Our results suggest that NAC and AA may effectively reduce or eliminate oxidative stress in cells exposed to VL-irradiated CQ/DMT following polymerization.
最近的研究表明，在叔胺（如N，N - 二甲基对甲苯胺，DMT）存在的情况下，可见光（VL）照射的樟脑醌（CQ）会产生引发自由基，这些自由基可能会与分子氧无差别地反应形成活性氧（ROS）。在本研究中，在永生化小鼠成牙骨质细胞系（OCCM.30）和永生化小鼠成纤维细胞系3T3 - 瑞士白化小鼠（3T3）中，评估了抗氧化剂N - 乙酰 - L - 半胱氨酸（NAC）和抗坏血酸（AA）降低由VL照射的CQ / DMT或VL照射的过氧化氢（H₂O₂）诱导的细胞内氧化应激的能力。用膜通透性染料2'，7' - 二氯二氢荧光素二乙酸酯（H₂DCF - DA）测量细胞内氧化应激。与未处理的VL照射细胞中的细胞内ROS水平相比，VL照射的CQ / DMT和VL照射的H₂O₂在两种细胞类型中均显著（p <0.001）提高了细胞内氧化水平。发现OCCM.30成牙骨质细胞对VL照射的CQ / DMT和VL照射的H₂O₂处理的敏感性几乎是3T3成纤维细胞的两倍。此外，10mM的NAC和10mM的AA均可消除两种细胞类型中由VL照射的CQ / DMT和VL照射的H₂O₂诱导的氧化应激。我们的结果表明，NAC和AA可能有效地降低或消除聚合后暴露于VL照射的CQ / DMT的细胞中的氧化应激。
