甲型流感病毒颗粒的形态和组成不受感染细胞中低水平M1和M2蛋白的影响。
The morphology and composition of influenza A virus particles are not affected by low levels of M1 and M2 proteins in infected cells.
作者信息
Bourmakina Svetlana V, García-Sastre Adolfo
机构信息
Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, Box 1124, New York, NY 10029, USA.
出版信息
J Virol. 2005 Jun;79(12):7926-32. doi: 10.1128/JVI.79.12.7926-7932.2005.
Abstract
We generated a recombinant influenza A virus (Mmut) that produced low levels of matrix (M1) and M2 proteins in infected cells. Mmut virus propagated to significantly lower titers than did wild-type virus in cells infected at low multiplicity. By contrast, virion morphology and incorporation of viral proteins and vRNAs into virus particles were similar to those of wild-type virus. We propose that a threshold amount of M1 protein is needed for the assembly of viral components into an infectious particle and that budding is delayed in Mmut virus-infected cells until sufficient levels of M1 protein accumulate at the plasma membrane.
摘要
我们构建了一种重组甲型流感病毒（Mmut），该病毒在感染细胞中产生低水平的基质（M1）蛋白和M2蛋白。在低感染复数感染的细胞中，Mmut病毒增殖至显著低于野生型病毒的滴度。相比之下，病毒粒子形态以及病毒蛋白和病毒RNA掺入病毒粒子的情况与野生型病毒相似。我们提出，病毒成分组装成感染性粒子需要一定阈值量的M1蛋白，并且在Mmut病毒感染的细胞中出芽延迟，直到足够水平的M1蛋白在质膜上积累。
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