Santos Júlio C, Pinto Icaro R G, Carvalho Walter, Mancilha Ismael M, Felipe Maria G A, Silva Silvio S
Faculdade de Engenharia Química de Lorena, Departamento de Biotecnologia, Rodovia Itajubá-Lorena, Km 74.5, C.P. 116, CEP, 12.600-970, Lorena/SP, Brazil.
Appl Biochem Biotechnol. 2005 Spring;121-124:673-83. doi: 10.1385/abab:122:1-3:0673.
Xylose-to-xylitol bioconversion was performed utilizing Candida guilliermondii immobilized in sugarcane bagasse and cultured in Erlenmeyer flasks using sugarcane bagasse hydrolysate as the source of xylose. Fermentations were carried out according to a factorial design, and the independent variables considered were treatment, average diameter, and amount of bagasse used as support for cell immobilization. By increasing the amount of support, the xylitol yield decreased, whereas the biomass yield increased. The diameter of the support did not influence xylitol production, and treatment of the bagasse with hexamethylene diamine prior to fermentation resulted in the highest amount of immobilized cells.
利用固定在甘蔗渣中的季也蒙毕赤酵母进行木糖到木糖醇的生物转化，并在锥形瓶中使用甘蔗渣水解液作为木糖来源进行培养。发酵按照析因设计进行，所考虑的自变量为处理方式、平均直径以及用作细胞固定化载体的甘蔗渣用量。通过增加载体用量，木糖醇产量降低，而生物量产量增加。载体直径对木糖醇生产没有影响，并且在发酵前用己二胺处理甘蔗渣可使固定化细胞数量最多。
