Fangkrathok Niramai, Junlatat Jintana, Umehara Kaoru, Noguchi Hiroshi, Sripanidkulchai Bungorn
Faculty of Agricultural Technology, Burapha University Sakaeo Campus, Sakaeo, 27160, Thailand.
J Nat Med. 2014 Apr;68(2):302-9. doi: 10.1007/s11418-013-0797-5. Epub 2013 Aug 15.
A polyhydroxyoctane, 6-methylheptane-1,2,3,4,5-pentaol (MHP), was first isolated from mycelia of the Thai edible mushroom Lentinus polychrous. MHP was evaluated for its cytotoxic and immunomodulatory effects in vitro. MHP was slightly cytotoxic to murine splenocytes but not to RAW264.7 cells or peripheral blood mononuclear cells. MHP decreased nitric oxide and intracellular O2 (-) production from lipopolysaccharide- and phorbol-12-myristate-13-acetate-activated RAW264.7 cells at levels of 78.98 ± 4.72 and 78.48 ± 2.41 % of controls, respectively. The mRNA expression of pro-inflammatory mediators, including iNOS, TNF-α, IL-1β, IL-6, COX-1 and COX-2, were significantly suppressed by MHP. In addition, MHP significantly increased the proliferation of phytohemagglutinin and pokeweed mitogen-induced splenocytes. These results indicate that MHP is able to modulate inflammatory responses and the proliferation of both T- and B-lymphocyte cells, suggesting that MHP may be a good natural immunomodulator.
一种多羟基辛烷，6-甲基庚烷-1,2,3,4,5-戊醇（MHP），最初是从泰国可食用蘑菇多毛香菇的菌丝体中分离出来的。对MHP的体外细胞毒性和免疫调节作用进行了评估。MHP对小鼠脾细胞有轻微的细胞毒性，但对RAW264.7细胞或外周血单个核细胞无细胞毒性。MHP使脂多糖和佛波醇-12-肉豆蔻酸酯-13-乙酸酯激活的RAW264.7细胞产生的一氧化氮和细胞内超氧阴离子（O2 (-)）分别降至对照组水平的78.98±4.72%和78.48±2.41%。MHP显著抑制了包括诱导型一氧化氮合酶（iNOS）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、环氧化酶-1（COX-1）和环氧化酶-2（COX-2）在内的促炎介质的mRNA表达。此外，MHP显著增加了植物血凝素和商陆有丝分裂原诱导的脾细胞增殖。这些结果表明，MHP能够调节炎症反应以及T淋巴细胞和B淋巴细胞的增殖，提示MHP可能是一种良好的天然免疫调节剂。
