从香港城市污水处理厂的活性污泥中分离出的细菌所产生的聚羟基脂肪酸酯（PHA）的微生物合成及其物理化学性质表征
Microbial synthesis and characterization of physiochemical properties of polyhydroxyalkanoates (PHAs) produced by bacteria isolated from activated sludge obtained from the municipal wastewater works in Hong Kong.
作者信息
Ma Tsz-Chun, Chan P L, Lawford H, Chua H, Lo W H, Yu Peter Hoifu
机构信息
State Key Lab of Chinese Med and Molecular Pharmacology, Shenzen, China.
出版信息
Appl Biochem Biotechnol. 2005 Spring;121-124:731-9.
The first objective of this study was the measurement of physical properties of P(3HB-co-3HV) copolymers with different (hydroxybutyrate) HB to (hydroxyvalerate) HV ratios produced by Bacillus cereus (TRY2) isolated from activated sludge. The 3HV PHBV copolymers were 0.05, 22.6, 39.2, 54.1, and 69.1 mol%, respectively. The second objective was to study possible waste water treatment and production of PHAs at the same time by B. cereus (TRY2) and Pseudomonas spp. (TOB17) (both were isolated from activated sludge), recombinant Bacillus DH5alpha, and a combination of the above three bacteria. The results were satisfactory; the maximum COD and TOC of the sewage sludge reduced were 53.5% and 67.5%, respectively.
本研究的首要目标是测定由从活性污泥中分离出的蜡样芽孢杆菌（TRY2）产生的、具有不同（羟基丁酸）HB与（羟基戊酸）HV比例的聚（3-羟基丁酸酯-co-3-羟基戊酸酯）共聚物的物理性质。3HV PHBV共聚物的比例分别为0.05、22.6、39.2、54.1和69.1摩尔%。第二个目标是研究蜡样芽孢杆菌（TRY2）和假单胞菌属（TOB17）（均从活性污泥中分离）、重组芽孢杆菌DH5α以及上述三种细菌的组合能否同时进行废水处理和聚羟基脂肪酸酯的生产。结果令人满意；污水污泥的最大化学需氧量（COD）和总有机碳（TOC）去除率分别为53.5%和67.5%。
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