Sun Lijie, Ryan David G, Zhou Mingyuan, Sun Tung-Tien, Lavker Robert M
Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA.
J Cell Physiol. 2006 Jan;206(1):103-11. doi: 10.1002/jcp.20433.
Using suppressive subtractive hybridization, we have identified a novel gene, which we named early epithelial differentiation associated (EEDA), which is uniquely associated with an early stage of stratified epithelial differentiation. In epidermis, esophageal epithelium, and tongue epithelium, EEDA mRNA, and antigen was abundant in suprabasal cells, but was barely detectable in more differentiated cells. Consistent with the limbal location of corneal epithelial stem cells, EEDA was expressed in basal corneal epithelial cells that are out of the stem cell compartment, as well as the suprabasal corneal epithelial cells. The strongest EEDA expression occurred in suprabasal precortical cells of mouse, bovine, and human anagen follicles. Developmental studies showed that the appearance of EEDA in embryonic mouse epidermis (E 15.5) coincided with morphological keratinization. Interestingly, EEDA expression is turned off when epithelia were perturbed by wounding and by cultivation under both low and high Ca2+ conditions. Our results indicate that EEDA is involved in the early stages of normal epithelial differentiation, and that EEDA is important for the "normal" differentiation pathway in a wide range of stratified epithelia.
利用抑制性消减杂交技术，我们鉴定出一个新基因，命名为早期上皮分化相关基因（EEDA），它与复层上皮分化的早期阶段独特相关。在表皮、食管上皮和舌上皮中，EEDA mRNA和抗原在上皮基底上层细胞中丰富，但在分化程度更高的细胞中几乎检测不到。与角膜上皮干细胞的角膜缘定位一致，EEDA在位于干细胞区室之外的角膜基底上皮细胞以及基底上层角膜上皮细胞中表达。EEDA在小鼠、牛和人类生长期毛囊的基底上层前皮质细胞中表达最强。发育研究表明，EEDA在胚胎小鼠表皮（E 15.5）中的出现与形态学角质化同时发生。有趣的是，当上皮受到创伤以及在低钙和高钙条件下培养时，EEDA表达关闭。我们的结果表明，EEDA参与正常上皮分化的早期阶段，并且EEDA对多种复层上皮的“正常”分化途径很重要。
