质粒pKC7：一种含有十个适合克隆DNA片段的限制性内切酶位点的载体。
Plasmid pKC7: a vector containing ten restriction endonuclease sites suitable for cloning DNA segments.
作者信息
Rao R N, Rogers S G
出版信息
Gene. 1979 Sep;7(1):79-82. doi: 10.1016/0378-1119(79)90044-1.
PMID:159209
Abstract
Plasmid pKC7, a derivative of pBR322, specifies resistance to both ampicillin and kanamycin. The DNA of this small plasmid (5.8 kb) contains unique sites for insertion of DNA cleaved with ten different restriction endonucleases. A detailed restriction endonuclease cleavage map is presented. The utility of this plasmid for cloning is discussed.
摘要
质粒pKC7是pBR322的衍生物，对氨苄青霉素和卡那霉素均具有抗性。这个小质粒（5.8 kb）的DNA含有用于插入用十种不同限制性内切酶切割的DNA的独特位点。给出了详细的限制性内切酶切割图谱。讨论了该质粒在克隆中的用途。
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