克隆细胞分裂素生物合成基因的鉴定。
Identification of a cloned cytokinin biosynthetic gene.
机构信息
Monsanto Company, 800 N. Lindbergh Boulevard, St. Louis, MO 63167.
出版信息
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4776-80. doi: 10.1073/pnas.81.15.4776.
Abstract
A small region of the Ti plasmid (the tmr locus), thought to be involved in phytohormone metabolism in Agrobacterium tumefaciens-transformed plant tissue, was cloned and expressed in Escherichia coli. By enzyme assay, the tmr locus was shown to encode isopentenyltransferase, an enzyme that catalyzes the first step in cytokinin biosynthesis.
摘要
Ti 质粒的一个小区域（tmr 基因座），被认为参与根癌农杆菌转化的植物组织中的植物激素代谢，已在大肠杆菌中克隆和表达。通过酶活性测定，tmr 基因座编码异戊烯基转移酶，该酶催化细胞分裂素生物合成的第一步。
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