Rodríguez-Pérez J, Rodríguez-Medina J R, García-Blanco M A, Hillyer G V
Department of Pathology, University of Puerto Rico School of Medicine, San Juan 00936-5067.
Exp Parasitol. 1992 Jun;74(4):400-7. doi: 10.1016/0014-4894(92)90202-l.
Immunization of mice with an antigenic polypeptide from Fasciola hepatica adult worms and having an apparent molecular mass of 12,000 Da (Fh12) has been shown to reduce the worm burden from challenge infection with Schistosoma mansoni by more than 50%. Moreover, mice infected with S. mansoni develop antibodies to Fh12 after 5-6 weeks of infection, indicating that this Fasciola-derived antigen is a cross-reactive, cross-protective protein. A lambda gt11 F. hepatica cDNA library was constructed from poly(A)+ RNA extracted from adult worms. A cDNA encoding a cross-reactive polypeptide (Fh15) was cloned by screening the F. hepatica lambda gt11 library with a monospecific, polyclonal rabbit antiserum against pure, native Fh12. The cDNA was sequenced and the predicted amino acid sequence revealed an open reading frame encoding a 132-amino-acid protein with a predicted molecular weight of 14,700 Da. This protein has significant homology to a 14-kDa S. mansoni fatty acid-binding protein. Comparison of the protective-inducing activity of recombinant Fh15 with that of purified Fh12 against schistosomes and Fasciola is warranted.
用来自肝片吸虫成虫的一种表观分子量为12,000道尔顿的抗原性多肽（Fh12）对小鼠进行免疫，已显示可使曼氏血吸虫攻击感染后的虫负荷减少50%以上。此外，感染曼氏血吸虫的小鼠在感染5至6周后会产生针对Fh12的抗体，这表明这种源自片形吸虫的抗原是一种交叉反应性、交叉保护性蛋白。从成虫提取的聚腺苷酸加尾RNA构建了一个λgt11肝片吸虫cDNA文库。通过用针对纯天然Fh12的单特异性多克隆兔抗血清筛选肝片吸虫λgt11文库，克隆了一个编码交叉反应性多肽（Fh15）的cDNA。对该cDNA进行测序，预测的氨基酸序列显示一个开放阅读框，编码一种132个氨基酸的蛋白质，预测分子量为14,700道尔顿。该蛋白质与一种14 kDa的曼氏血吸虫脂肪酸结合蛋白具有显著同源性。有必要比较重组Fh15与纯化的Fh12对血吸虫和片形吸虫的诱导保护活性。
